NMR localization of the O-mycoloylation on PorH, a channel forming peptide from Corynebacterium glutamicum
| العنوان: | NMR localization of the O-mycoloylation on PorH, a channel forming peptide from Corynebacterium glutamicum |
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| المؤلفون: | Mamadou Daffé, Parthasarathi Rath, Maryelle Tropis, Pascal Demange, Olivier Saurel, Alain Milon |
| المساهمون: | Institut de pharmacologie et de biologie structurale (IPBS), Centre National de la Recherche Scientifique (CNRS)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées |
| المصدر: | FEBS Letters FEBS Letters, Wiley, 2013, 587 (22), pp.3687-3691. ⟨10.1016/j.febslet.2013.09.032⟩ |
| بيانات النشر: | HAL CCSD, 2013. |
| سنة النشر: | 2013 |
| مصطلحات موضوعية: | MALDI-TOF, Stereochemistry, HSQC-TOCSY, Lipoylation, [SDV]Life Sciences [q-bio], Molecular Sequence Data, Biophysics, Porins, Peptide, Biochemistry, Corynebacterium glutamicum, 03 medical and health sciences, Structural Biology, Genetics, Post translational modification, Serine, Amino Acid Sequence, Site-directed mutagenesis, Molecular Biology, Nuclear Magnetic Resonance, Biomolecular, Ion channel, ComputingMilieux_MISCELLANEOUS, 030304 developmental biology, chemistry.chemical_classification, 0303 health sciences, Mycolic acid, 030306 microbiology, Chemistry, Protein primary structure, Cell Biology, HSQC-NOESY, Mycolic Acids, Porin, Proton NMR, Bacterial outer membrane, Protein Processing, Post-Translational |
| الوصف: | PorH and PorA are two small peptides that, in complex, form a voltage-dependent ion channel in the outer membrane of Corynebacterium glutamicum. Specific post-translational modifications on PorA and PorH are required for the formation of a functional ion channel. The assignment of PorH proton NMR chemical shifts in DMSO, allowed identifying unambiguously the exact position of the PorH O-mycoloylation on Ser 56 side chain. This was further confirmed by site directed mutagenesis and mass spectrometry. Together with the previously published localization of PorA mycoloylation, this provides the complete primary structure characterization of this outer membrane porin. |
| اللغة: | English |
| تدمد: | 0014-5793 1873-3468 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0b3aebec7fb208ed086a1ae0cc313398 https://hal.archives-ouvertes.fr/hal-03100885 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....0b3aebec7fb208ed086a1ae0cc313398 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 00145793 18733468 |
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