Sodium starch octenyl succinate facilitated the production of water-soluble yellow pigments in Monascus ruber fermentation
| العنوان: | Sodium starch octenyl succinate facilitated the production of water-soluble yellow pigments in Monascus ruber fermentation |
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| المؤلفون: | Hai-Qing Liu, Shan-Zhong Yang, Zhenqiang Wu, Xiao-Fei Tian, Zhen-Feng Huang |
| المصدر: | Applied Microbiology and Biotechnology. 105:6691-6706 |
| بيانات النشر: | Springer Science and Business Media LLC, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | Membrane permeability, Dehydrogenase, Applied Microbiology and Biotechnology, Pigment, chemistry.chemical_compound, Biosynthesis, Food science, Mycelium, biology, Chemistry, Sodium, Water, Starch, Succinates, Pigments, Biological, General Medicine, Monascus, biology.organism_classification, body regions, visual_art, Fermentation, visual_art.visual_art_medium, NAD+ kinase, Biotechnology |
| الوصف: | Natural water-soluble Monascus pigments (WSMPs) have been in increasing demand but have not been able to achieve industrial production due to the low production rate. This study aimed to improve the biosynthesis and secretion of extracellular yellow pigments (EYPs) through submerged fermentation with Monascus ruber CGMCC 10,910 supplemented with sodium starch octenyl succinate (OSA-SNa). The results demonstrated that the yield was 69.68% and 48.89% higher than that without OSA-SNa in conventional fermentation (CF) and extractive fermentation (EF), respectively. The mainly increased EYP components were Y3 and Y4 in CF, but they were mainly Y1 and Y2 as well as secreted intracellular pigments, including Y5, Y6, O1, and O2, in EF. Scanning electron microscopy analysis revealed that the mycelium presented an uneven surface profile with obvious wrinkles and small fragments with OSA-SNa. It was found that a higher unsaturated/saturated fatty acids ratio in the cell membrane resulted in increased permeability and facilitated the export of intracellular yellow pigments into the broth with OSA-SNa treatment. In addition, a higher NAD+/NADH ratio and glucose-6-phosphate dehydrogenase activity provided a reducing condition for yellow pigment biosynthesis. Gene expression analysis showed that the expression levels of the key genes for yellow pigment biosynthesis were significantly upregulated by OSA-SNa. This study provides an effective strategy to promote the production of WSMPs by microparticle-enhanced cultivation using OSA-SNa. KEY POINTS: • OSA-SNa addition facilitated the production of Monascus yellow pigments. • Mycelial morphology and membrane permeability were affected by OSA-SNa. • The key gene expression of yellow pigments was upregulated. |
| تدمد: | 1432-0614 0175-7598 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0bb5b84bcaab691ed34fc539e3a3634c https://doi.org/10.1007/s00253-021-11512-7 |
| حقوق: | CLOSED |
| رقم الأكسشن: | edsair.doi.dedup.....0bb5b84bcaab691ed34fc539e3a3634c |
| قاعدة البيانات: | OpenAIRE |
Find this article in full text from Springer Verlag. | تدمد: | 14320614 01757598 |
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