Responses of the marine diatom Thalassiosira pseudonana to changes in CO2 concentration: a proteomic approach
| العنوان: | Responses of the marine diatom Thalassiosira pseudonana to changes in CO2 concentration: a proteomic approach |
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| المؤلفون: | Stephen C. Maberly, Sabrina Lignon, Romain Clement, Carine Puppo, Brigitte Gontero, Pascal Mansuelle, Yann Denis, Erik Jensen, Régine Lebrun, Matthieu Pophillat |
| المساهمون: | Bioénergétique et Ingénierie des Protéines (BIP ), Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS), Aix Marseille Université (AMU), Plateforme Protéomique [Marseille], Institut de Microbiologie de la Méditerranée (IMM), Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS)-Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS), Centre d'Immunologie de Marseille - Luminy (CIML), Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Aix Marseille Université (AMU), Lake Ecosystems Group [Lancaster, U.K.] (Centre for Ecology & Hydrology), Lancaster Environment Centre [Lancaster, U.K.], Centre National de la Recherche Scientifique (CNRS)-Aix Marseille Université (AMU), Aix Marseille Université (AMU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), AZZOPARDI, LAURE |
| المصدر: | Scientific Reports Scientific Reports, Nature Publishing Group, 2017, 7 (42333 ), ⟨10.1038/srep42333⟩ Scientific Reports, 2017, 7 (42333 ), ⟨10.1038/srep42333⟩ |
| بيانات النشر: | Springer Science and Business Media LLC, 2017. |
| سنة النشر: | 2017 |
| مصطلحات موضوعية: | Proteomics, 0301 basic medicine, Aquatic Organisms, Thalassiosira pseudonana, Models, Biological, Article, 03 medical and health sciences, Algae, Carbonic anhydrase, Botany, Electrophoresis, Gel, Two-Dimensional, Amino Acid Sequence, RNA, Messenger, 14. Life underwater, [SDV.MP] Life Sciences [q-bio]/Microbiology and Parasitology, Diatoms, chemistry.chemical_classification, Genome, Multidisciplinary, biology, RuBisCO, Proteins, Carbon Dioxide, biology.organism_classification, Enzyme assay, Marine Sciences, Chloroplast, Metabolic pathway, [SDV.EE.BIO] Life Sciences [q-bio]/Ecology, environment/Bioclimatology, [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology, 030104 developmental biology, Enzyme, Gene Expression Regulation, Solubility, chemistry, Biochemistry, biology.protein, [SDV.EE.BIO]Life Sciences [q-bio]/Ecology, environment/Bioclimatology |
| الوصف: | The concentration of CO2 in many aquatic systems is variable, often lower than the KM of the primary carboxylating enzyme Rubisco, and in order to photosynthesize efficiently, many algae operate a facultative CO2 concentrating mechanism (CCM). Here we measured the responses of a marine diatom, Thalassiosira pseudonana, to high and low concentrations of CO2 at the level of transcripts, proteins and enzyme activity. Low CO2 caused many metabolic pathways to be remodeled. Carbon acquisition enzymes, primarily carbonic anhydrase, stress, degradation and signaling proteins were more abundant while proteins associated with nitrogen metabolism, energy production and chaperones were less abundant. A protein with similarities to the Ca2+/ calmodulin dependent protein kinase II_association domain, having a chloroplast targeting sequence, was only present at low CO2. This protein might be a specific response to CO2 limitation since a previous study showed that other stresses caused its reduction. The protein sequence was found in other marine diatoms and may play an important role in their response to low CO2 concentration. |
| وصف الملف: | text; application/pdf |
| تدمد: | 2045-2322 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0c1bef6ba42d3938d6766e48fa855d4d https://doi.org/10.1038/srep42333 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....0c1bef6ba42d3938d6766e48fa855d4d |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 20452322 |
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