Immunohistochemical and in situ hybridization studies of the liver and kidney in human leptospirosis
| العنوان: | Immunohistochemical and in situ hybridization studies of the liver and kidney in human leptospirosis |
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| المؤلفون: | Ana Maria Gonçalves da Silva, Silvia Vanessa Lourenço, T. De Brito, Dirce Mary Correia Lima, Venâncio Avancini Ferreira Alves, Luis F. Menezes |
| المصدر: | Virchows Archiv. 448:576-583 |
| بيانات النشر: | Springer Science and Business Media LLC, 2006. |
| سنة النشر: | 2006 |
| مصطلحات موضوعية: | Adult, DNA, Bacterial, Male, Pathology, medicine.medical_specialty, Guinea Pigs, In situ hybridization, Kidney, Pathology and Forensic Medicine, Antigen, medicine, Animals, Humans, Leptospirosis, Molecular Biology, In Situ Hybridization, biology, Cell Membrane, Cell Biology, General Medicine, Middle Aged, Apical membrane, Cadherins, medicine.disease, Immunohistochemistry, Staining, medicine.anatomical_structure, Liver, biology.protein, Female, Antibody |
| الوصف: | An in situ hybridization (ISH) assay for the detection of leptospiral DNA in tissues was described and its diagnostic and pathogenetic usefulness in combination with immunohistochemistry (IHC) was evaluated in formalin-fixed, paraffin-embedded liver and kidney samples from human fatal cases of leptospirosis. IHC assays with anti-E-cadherin antibodies assessed the liver-plate disarray frequently observed in leptospirosis. Immunohistochemistry detected leptospiral antigen (LAg) in macrophages, both in human liver and kidney. In guinea pigs, in addition to these findings, staining on cell membranes of hepatocytes and, occasionally, in apical membrane of kidney tubular cells was demonstrated. Positive ISH signal was observed chiefly in the nuclei of human hepatocytes and in the cytoplasm and nuclei of liver cells of experimentally infected guinea pigs. Loss of E-cadherin membrane expression is associated with liver-plate disarray. These findings were discussed in the contention that, in leptospirosis, cell membrane damage might be important for the pathogenesis of the disease. Finally, it was suggested that both IHC and/or ISH might be used for both diagnostic and research purposes. |
| تدمد: | 1432-2307 0945-6317 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0c2a48c5dc49a9020f139a0ec43a975c https://doi.org/10.1007/s00428-006-0163-z |
| حقوق: | CLOSED |
| رقم الأكسشن: | edsair.doi.dedup.....0c2a48c5dc49a9020f139a0ec43a975c |
| قاعدة البيانات: | OpenAIRE |
Find this article in full text from Springer Verlag. | تدمد: | 14322307 09456317 |
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