MMP-9 Knockdown Inhibits Oral Squamous Cell Carcinoma Lymph Node Metastasis in the Nude Mouse Tongue-Xenografted Model through the RhoC/Src Pathway
| العنوان: | MMP-9 Knockdown Inhibits Oral Squamous Cell Carcinoma Lymph Node Metastasis in the Nude Mouse Tongue-Xenografted Model through the RhoC/Src Pathway |
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| المؤلفون: | Ying Su, Feng Gao, Jinlin Wen, Xinyan Zhang, Suhong Chen, Panpan Yin, Yanlin Wu |
| المصدر: | Analytical Cellular Pathology, Vol 2021 (2021) Analytical Cellular Pathology (Amsterdam) |
| بيانات النشر: | Hindawi Limited, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | 0301 basic medicine, Cancer Research, Article Subject, Axillary lymph nodes, Angiogenesis, RhoC, Mice, Nude, Pathology and Forensic Medicine, Metastasis, Mice, 03 medical and health sciences, 0302 clinical medicine, Nude mouse, medicine, Animals, Humans, Neoplasm Invasiveness, RC254-282, Mice, Inbred BALB C, Neovascularization, Pathologic, QH573-671, biology, Squamous Cell Carcinoma of Head and Neck, Neoplasms. Tumors. Oncology. Including cancer and carcinogens, Cell migration, Cell Biology, General Medicine, medicine.disease, biology.organism_classification, stomatognathic diseases, src-Family Kinases, 030104 developmental biology, medicine.anatomical_structure, Matrix Metalloproteinase 9, rhoC GTP-Binding Protein, Gene Knockdown Techniques, Lymphatic Metastasis, 030220 oncology & carcinogenesis, Cancer cell, biology.protein, Cancer research, Heterografts, Molecular Medicine, Mouth Neoplasms, Lymph, Cytology, Signal Transduction, Research Article |
| الوصف: | Oral squamous cell carcinoma (OSCC) is one of the most common types of cancers in developing countries. A major contributor to the high mortality rate of OSCC is the tendency of oral cancer cells to metastasize to lymph nodes around the head and neck during the early stages of cancer development. Matrix metalloproteinase 9 (MMP-9), an endopeptidase, can degrade the extracellular matrix and basement membrane and plays a key role in tumor invasion and metastasis. In vitro, cell migration ability was conducted by scratching assays. We also investigated the interaction abilities between OSCC cells and vascular endothelial cells (ECs) by an adhesion assay and transendothelial migration assay. And we established a BALB/c nude mouse tongue-xenografted metastasis model to investigate the role of MMP-9 and explore its potential underlying mechanism in OSCC growth, lymph node metastasis, and angiogenesis in vivo. The results showed that knockdown of MMP-9 could significantly suppress OSCC cell migration, proliferation, interactions between endothelial cells, xenografted tumor growth, and angiogenesis and simultaneously markedly inhibited OSCC cell metastasis to mouse lymphonodi cervicales superficiales, axillary lymph nodes, and even distant inguinal lymph nodes. Mechanistic studies revealed that knockdown of MMP-9 also led to a decreased expression of RhoC, Src, and F-actin by RT-PCR, western blotting, and immunohistochemistry. And the bioinformatic analysis showed that MMP-9, RhoC, and Src mRNA expression was positively and linearly correlated in OSCC on TCGA database. Together, our findings indicated that MMP-9 plays a very important role in OSCC growth, migration, angiogenesis, and lymph node metastasis, and its potential mechanism may be mediated by RhoC and Src gene expression. |
| وصف الملف: | text/xhtml |
| تدمد: | 2210-7185 2210-7177 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0c5ad3e452405bc3d188f5d708eedd4e https://doi.org/10.1155/2021/6683391 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....0c5ad3e452405bc3d188f5d708eedd4e |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 22107185 22107177 |
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