Reconstitution of the Jasmonate Signaling Pathway in Plant Protoplasts
| العنوان: | Reconstitution of the Jasmonate Signaling Pathway in Plant Protoplasts |
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| المؤلفون: | Li-Jun Huang, Corinna Thurow, Joachim F. Uhrig, Christiane Gatz, Ning Li |
| المصدر: | Cells Volume 8 Issue 12 |
| بيانات النشر: | MDPI AG, 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | signaling pathway, 0106 biological sciences, 0301 basic medicine, Arabidopsis, Repressor, Cyclopentanes, transient gene expression, 01 natural sciences, Article, 03 medical and health sciences, chemistry.chemical_compound, protoplasts, Gene Expression Regulation, Plant, Arabidopsis thaliana, Oxylipins, Psychological repression, Transcription factor, biology, Arabidopsis Proteins, Basic Helix-Loop-Helix Leucine Zipper Transcription Factors, Jasmonic acid, jasmonic acid, General Medicine, biology.organism_classification, Fusion protein, Cell biology, Repressor Proteins, 030104 developmental biology, chemistry, Signal transduction, Corepressor, Signal Transduction, 010606 plant biology & botany |
| الوصف: | The phytohormone jasmonic acid (JA) plays an important role in various plant developmental processes and environmental adaptations. The JA signaling pathway has been well-elucidated in the reference plant Arabidopsis thaliana. It starts with the perception of the active JA derivative, jasmonoyl-isoleucine (JA-Ile), by the F-box protein COI1 which is part of the E3-ligase SCFCOI1. Binding of JA-Ile enables the interaction between COI1 and JAZ repressor proteins. Subsequent degradation of JAZ proteins leads to the activation of transcription factors like e.g., MYC2. Here we demonstrate that the pathway can be reconstituted in transiently transformed protoplasts. Analysis of the stability of a JAZ1-fLuc fusion protein as a function of COI1 transiently expressed in coi1 protoplasts allows structure function analysis of both JAZs and COI1. Using this system, we found that conserved cysteines in COI1 influence steady state COI1 protein levels. Using a luciferase reporter gene under the control of the JAZ1 promoter enable to address those features of JAZ1 that are required for MYC2 repression. Interestingly, the conserved TIFY-motif previously described to interact with NINJA to recruit the corepressor TOPLESS is not necessary for repression. This result is in favor of the alternative repression mode that proposes a direct competition between repressive JAZs and promotive MEDIATOR25 at MYC2. Finally, using protoplasts from the aos coi1 double mutant, which is deficient in JA synthesis and perception, we provide a system that has the potential to study the activity of different COI1 variants in the presence of different ligands. |
| وصف الملف: | application/pdf |
| تدمد: | 2073-4409 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0d2f42158314be538ccc1f817e676499 https://doi.org/10.3390/cells8121532 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....0d2f42158314be538ccc1f817e676499 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 20734409 |
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