The Susceptibility of Pseudomonas aeruginosa Strains from Cystic Fibrosis Patients to Bacteriophages
| العنوان: | The Susceptibility of Pseudomonas aeruginosa Strains from Cystic Fibrosis Patients to Bacteriophages |
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| المؤلفون: | Christine Pourcel, Arsher Cablanmian, Yann Blouin, Guillaume Loukou, Christiane Essoh, Gilles Vergnaud, Serge Lathro, Hoang Vu Thien, Elizabeth Kutter |
| المساهمون: | Institut de génétique et microbiologie [Orsay] (IGM), Université Paris-Sud - Paris 11 (UP11)-Centre National de la Recherche Scientifique (CNRS) |
| المصدر: | PLoS ONE PLoS ONE, Public Library of Science, 2013, 8 (4), pp.e60575. ⟨10.1371/journal.pone.0060575⟩ PLoS ONE, Vol 8, Iss 4, p e60575 (2013) |
| بيانات النشر: | HAL CCSD, 2013. |
| سنة النشر: | 2013 |
| مصطلحات موضوعية: | Bacterial Diseases, Cystic Fibrosis, medicine.medical_treatment, Applied Microbiology, Antibiotics, Viral Plaque Assay, medicine.disease_cause, Genome, Autosomal Recessive, Gene Order, CRISPR, Bacteriophages, Clustered Regularly Interspaced Short Palindromic Repeats, Genetics, 0303 health sciences, Multidisciplinary, Infectious Diseases, [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology, Lytic cycle, Medical Microbiology, Pseudomonas aeruginosa, Medicine, Research Article, Biotechnology, Phage therapy, Infectious Disease Control, medicine.drug_class, Science, Genome, Viral, Biology, Microbiology, DNA sequencing, 03 medical and health sciences, Microbial Control, medicine, Humans, Pseudomonas Infections, [SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology, Microbial Pathogens, 030304 developmental biology, Phage typing, Clinical Genetics, 030306 microbiology, Human Genetics, Bacteriology, CRISPR-Cas Systems |
| الوصف: | International audience; Phage therapy may become a complement to antibiotics in the treatment of chronic Pseudomonas aeruginosa infection. To design efficient therapeutic cocktails, the genetic diversity of the species and the spectrum of susceptibility to bacteriophages must be investigated. Bacterial strains showing high levels of phage resistance need to be identified in order to decipher the underlying mechanisms. Here we have selected genetically diverse P. aeruginosa strains from cystic fibrosis patients and tested their susceptibility to a large collection of phages. Based on plaque morphology and restriction profiles, six different phages were purified from "pyophage", a commercial cocktail directed against five different bacterial species, including P. aeruginosa. Characterization of these phages by electron microscopy and sequencing of genome fragments showed that they belong to 4 different genera. Among 47 P. aeruginosa strains, 13 were not lysed by any of the isolated phages individually or by pyophage. We isolated two new phages that could lyse some of these strains, and their genomes were sequenced. The presence/absence of a CRISPR-Cas system (Clustered Regularly Interspaced Short Palindromic Repeats and Crisper associated genes) was investigated to evaluate the role of the system in phage resistance. Altogether, the results show that some P. aeruginosa strains cannot support the growth of any of the tested phages belonging to 5 different genera, and suggest that the CRISPR-Cas system is not a major defence mechanism against these lytic phages. |
| اللغة: | English |
| تدمد: | 1932-6203 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0d326fea22c1ef22102375420801d696 https://hal.archives-ouvertes.fr/hal-00822766 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....0d326fea22c1ef22102375420801d696 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19326203 |
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