التفاصيل البيبلوغرافية
| العنوان: |
Differential metabolism of benzo[alpha]pyrene in vitro by human placental tissues exposed to active maternal cigarette smoke |
| المؤلفون: |
You Lan Li, Mrinal K. Sanyal |
| المصدر: |
Birth defects research. Part B, Developmental and reproductive toxicology. 80(1) |
| سنة النشر: |
2007 |
| مصطلحات موضوعية: |
Embryology, medicine.medical_specialty, Health, Toxicology and Mutagenesis, Metabolite, Placenta, Toxicology, chemistry.chemical_compound, DNA Adducts, Pregnancy, Internal medicine, Microsomes, medicine, Benzo(a)pyrene, Cytochrome P-450 CYP1A1, Humans, Benzopyrene Hydroxylase, chemistry.chemical_classification, Smoke, Smoking, Trophoblast, Metabolism, Immunohistochemistry, In vitro, Enzyme, Endocrinology, medicine.anatomical_structure, chemistry, Microsome, Female, Xenobiotic, Developmental Biology |
| الوصف: |
OBJECTIVES: Generation of different metabolites and DNA-adduct(s) of metabolites of benzo[α]pyrene (B[α]P) in vitro by placental tissues (microsomes) of mothers who actively smoked cigarettes (tobacco) and those who did not smoke were analyzed to determine the variability in metabolism of the B[α]P substrate among individual placental samples. METHODS: Overall B[α]P metabolism was assayed by alkaline aqueous extraction of metabolites, and reactive metabolites by DNA adducts of B[α]P-metabolites produced by salmon sperm DNA added to the incubation mixtures of the substrate and microsomes of exposed- and unexposed-placentas to maternal cigarette smoke. Array of B[α]P-metabolites produced by the same incubations were identified by high pressure liquid chromatography of the aqueous extracts. RESULTS: Subsets of smoke-exposed placentas assessed by cluster analysis had augmented metabolic activity, others did not respond to smoke exposure. CYP1A1 expression in trophoblast cells analyzed by immunohistochemistry did not correlate with smoke exposure. The DNA-adducts generated was variable, regardless of verbally reported levels of maternal exposure. The amounts of different B[α]P-metabolites produced by individual samples matched for similar levels of exposure during pregnancy by self-reported smoking (1 pack/day) were also not comparable. Metabolism of B[α]P into different metabolites, and production of toxic DNA adducts from metabolites in vitro by human placenta were variable and unrelated to the extent of smoke exposure. CONCLUSIONS: The metabolic characteristic of human placenta for xenobiotic exposure substrates is based on the expression and function of diverse enzymes, and such metabolism exhibited inter-individual variation for toxic metabolite production or detoxification of the substrates in response to maternal smoke exposure. Birth Defects Res (Part B) 80:49–56, 2007. © 2007 Wiley-Liss, Inc. |
| تدمد: |
1542-9733 |
| URL الوصول: |
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0d74526b4d8ab0f8aaa61a43bba6007a
https://pubmed.ncbi.nlm.nih.gov/17294456 |
| حقوق: |
CLOSED |
| رقم الأكسشن: |
edsair.doi.dedup.....0d74526b4d8ab0f8aaa61a43bba6007a |
| قاعدة البيانات: |
OpenAIRE |
