Visualizing multistep elevator-like transitions of a nucleoside transporter
| العنوان: | Visualizing multistep elevator-like transitions of a nucleoside transporter |
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| المؤلفون: | Marscha Hirschi, Seok-Yong Lee, Zachary Lee Johnson |
| المصدر: | Nature |
| سنة النشر: | 2017 |
| مصطلحات موضوعية: | 0301 basic medicine, Models, Molecular, Elevator, Movement, Protein domain, Nucleoside Transport Proteins, Nucleoside transporter, Crystallography, X-Ray, Ligands, Models, Biological, Article, Domain (software engineering), Concentrative nucleoside transporter, 03 medical and health sciences, Protein Domains, Cysteine, Binding site, Uridine, Multidisciplinary, Binding Sites, biology, Chemistry, Elevators and Escalators, Transport protein, Crystallography, 030104 developmental biology, Membrane, Mutation, biology.protein, Biophysics, Crystallization, Neisseria |
| الوصف: | Membrane transporters move substrates across the membrane by alternating access of their binding sites between the opposite sides of the membrane. An emerging model of this process is the elevator mechanism, in which a substrate-binding transport domain moves a large distance across the membrane. This mechanism has been characterized by a transition between two states, but the conformational path that leads to the transition is not yet known, largely because the available structural information has been limited to the two end states. Here we present crystal structures of the inward-facing, intermediate, and outward-facing states of a concentrative nucleoside transporter from Neisseria wadsworthii. Notably, we determined the structures of multiple intermediate conformations, in which the transport domain is captured halfway through its elevator motion. Our structures present a trajectory of the conformational transition in the elevator model, revealing multiple intermediate steps and state-dependent conformational changes within the transport domain that are associated with the elevator-like motion. |
| اللغة: | English |
| تدمد: | 1476-4687 0028-0836 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0d86185dd3a7f81134c75d763baca01f http://europepmc.org/articles/PMC5567992 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....0d86185dd3a7f81134c75d763baca01f |
| قاعدة البيانات: | OpenAIRE |
Find this article in full text from Springer Verlag. | تدمد: | 14764687 00280836 |
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