Fine mapping of powdery mildew and stripe rust resistance genes Pm5V/Yr5V transferred from Dasypyrum villosum into wheat without yield penalty
| العنوان: | Fine mapping of powdery mildew and stripe rust resistance genes Pm5V/Yr5V transferred from Dasypyrum villosum into wheat without yield penalty |
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| المؤلفون: | Ruiqi Zhang, Chuntian Lu, Xiangru Meng, Yali Fan, Jie Du, Runran Liu, Yigao Feng, Liping Xing, Petr Cápal, Kateřina Holušová, Jaroslav Doležel, Yiwei Wang, Huanqing Mu, Bingxiao Sun, Fu Hou, Ruonan Yao, Chuanxi Xiong, Yang Wang, Peidu Chen, Aizhong Cao |
| المصدر: | Theoretical and Applied Genetics. 135:3629-3642 |
| بيانات النشر: | Springer Science and Business Media LLC, 2022. |
| سنة النشر: | 2022 |
| مصطلحات موضوعية: | Plant Breeding, Basidiomycota, Genetics, General Medicine, Poaceae, Agronomy and Crop Science, Triticum, Disease Resistance, Plant Diseases, Biotechnology |
| الوصف: | The novel wheat powdery mildew and stripe rust resistance genes Pm5V/Yr5V are introgressed from Dasypyrum villosum and fine mapped to a narrowed region in 5VS, and their effects on yield-related traits were characterized. The powdery mildew and stripe rust seriously threaten wheat production worldwide. Dasypyrum villosum (2n = 2x = 14, VV), a relative of wheat, is a valuable resource of resistance genes for wheat improvement. Here, we describe a platform for rapid introgression of the resistance genes from D. villosum into the wheat D genome. A complete set of new wheat-D. villosum V (D) disomic substitution lines and 11 D/V Robertsonian translocation lines are developed and characterized by molecular cytogenetic method. A new T5DL·5V#5S line NAU1908 shows resistance to both powdery mildew and stripe rust, and the resistances associated with 5VS are confirmed to be conferred by seedling resistance gene Pm5V and adult-plant resistance gene Yr5V, respectively. We flow-sort chromosome arm 5VS and sequence it using the Illumina NovaSeq 6000 system that allows us to generate 5VS-specific markers for genetic mapping of Pm5V/Yr5V. Fine mapping shows that Pm5V and Yr5V are closely linked and the location is narrowed to an approximately 0.9 Mb region referencing the sequence of Chinese Spring 5DS. In this region, a NLR gene in scaffold 24,874 of 5VS orthologous to TraesCS5D02G044300 is the most likely candidate gene for Pm5V. Soft- and hard-grained T5DL·5V#5S introgressions confer resistance to both powdery mildew and stripe rust in diverse wheat genetic backgrounds without yield penalty. Meanwhile, significant decrease in plant height and increase in yield were observed in NIL-5DL·5V#5S compared with that in NIL-5DL·5DS. These results indicate that Pm5V/Yr5V lines might have the potential value to facilitate wheat breeding for disease resistance. |
| تدمد: | 1432-2242 0040-5752 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0dc688dd9ceb6724ca60dfe86eba1d8f https://doi.org/10.1007/s00122-022-04206-9 |
| حقوق: | CLOSED |
| رقم الأكسشن: | edsair.doi.dedup.....0dc688dd9ceb6724ca60dfe86eba1d8f |
| قاعدة البيانات: | OpenAIRE |
Find this article in full text from Springer Verlag. | تدمد: | 14322242 00405752 |
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