Artificial Slanted Nanocilia Array as a Mechanotransducer for Controlling Cell Polarity
| العنوان: | Artificial Slanted Nanocilia Array as a Mechanotransducer for Controlling Cell Polarity |
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| المؤلفون: | Noo Li Jeon, Pilnam Kim, Byung Soo Kim, Hoon Eui Jeong, Min Sung Kim, Daeshik Kang, Sang Moon Kim, Hong Nam Kim, Kyung-Jin Jang, Kahp-Yang Suh, Ilkyoo Koh, Segeun Jang, Yoonmi Hong, Jung-Youn Shin |
| المصدر: | ACS Nano. 11:730-741 |
| بيانات النشر: | American Chemical Society (ACS), 2017. |
| سنة النشر: | 2017 |
| مصطلحات موضوعية: | 0301 basic medicine, Collagen type, Matrigel, Materials science, biology, business.industry, General Engineering, General Physics and Astronomy, 02 engineering and technology, equipment and supplies, 021001 nanoscience & nanotechnology, Polarization (waves), Fibronectin, 03 medical and health sciences, 030104 developmental biology, Optics, Bending stiffness, Cell polarity, biology.protein, Biophysics, Directionality, General Materials Science, 0210 nano-technology, business, Anisotropy |
| الوصف: | We present a method to induce cell directional behavior using slanted nanocilia arrays. NIH-3T3 fibroblasts demonstrated bidirectional polarization in a rectangular arrangement on vertical nanocilia arrays and exhibited a transition from a bidirectional to a unidirectional polarization pattern when the angle of the nanocilia was decreased from 90° to 30°. The slanted nanocilia guided and facilitated spreading by allowing the cells to contact the sidewalls of the nanocilia, and the directional migration of the cells opposed the direction of the slant due to the anisotropic bending stiffness of the slanted nanocilia. Although the cells recognized the underlying anisotropic geometry when the nanocilia were coated with fibronectin, collagen type I, and Matrigel, the cells lost their directionality when the nanocilia were coated with poly-d-lysine and poly-l-lysine. Furthermore, although the cells recognized geometrical anisotropy on fibronectin coatings, pharmacological perturbation of PI3K-Rac signaling hindered the directional elongation of the cells on both the slanted and vertical nanocilia. Furthermore, myosin light chain II was required for the cells to obtain polarized morphologies. These results indicated that the slanted nanocilia array provided anisotropic contact guidance cues to the interacting cells. The polarization of cells was controlled through two steps: the recognition of underlying geometrical anisotropy and the subsequent directional spreading according to the guidance cues. |
| تدمد: | 1936-086X 1936-0851 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0e3015f09ed675a0441d6b3851755612 https://doi.org/10.1021/acsnano.6b07134 |
| رقم الأكسشن: | edsair.doi.dedup.....0e3015f09ed675a0441d6b3851755612 |
| قاعدة البيانات: | OpenAIRE |
Find this issue from the American Chemical Society | تدمد: | 1936086X 19360851 |
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