Human apoA-I increases macrophage foam cell derived PLTP activity without affecting the PLTP mass
| العنوان: | Human apoA-I increases macrophage foam cell derived PLTP activity without affecting the PLTP mass |
|---|---|
| المؤلفون: | Marius R. Robciuc, Matti Jauhiainen, Christian Ehnholm, Jari Metso, Anca Sima |
| المساهمون: | Institute for Molecular Medicine Finland |
| المصدر: | Lipids in Health and Disease, Vol 9, Iss 1, p 59 (2010) Lipids in Health and Disease |
| سنة النشر: | 2010 |
| مصطلحات موضوعية: | Apolipoprotein E, Protein Denaturation, Hot Temperature, CHOLESTEROL EFFLUX, Endocrinology, Diabetes and Metabolism, Clinical Biochemistry, Cell, 030204 cardiovascular system & hematology, FORMS, chemistry.chemical_compound, 0302 clinical medicine, Endocrinology, ATHEROSCLEROTIC LESIONS, Phospholipid transfer protein, BINDING, polycyclic compounds, Phospholipid Transfer Proteins, Bovine serum albumin, lcsh:RC620-627, Cells, Cultured, Foam cell, 0303 health sciences, biology, A-I, DEFICIENCY, lcsh:Nutritional diseases. Deficiency diseases, medicine.anatomical_structure, Biochemistry, SECRETION, lipids (amino acids, peptides, and proteins), PHOSPHOLIPID TRANSFER PROTEIN, Phospholipid, Protective Agents, Apolipoproteins E, 03 medical and health sciences, medicine, Humans, Secretion, HUMAN-PLASMA, 030304 developmental biology, Biochemistry, medical, Apolipoprotein A-I, Research, Biochemistry (medical), nutritional and metabolic diseases, MICE, chemistry, biology.protein, 3111 Biomedicine, Foam Cells |
| الوصف: | Background phospholipid transfer protein (PLTP) plays important roles in lipoprotein metabolism and atherosclerosis and is expressed by macrophages and macrophage foam cells (MFCs). The aim of the present study was to determine whether the major protein from HDL, apoA-I, affects PLTP derived from MFCs. Results as cell model we used human THP-1 monocytes incubated with acetylated LDL, to generate MFC. The addition of apoA-I to the cell media increased apoE secretion from the cells, in a concentration dependent fashion, without affecting cellular apoE levels. In contrast, apoA-I had no effect on PLTP synthesis and secretion, but strongly induced the PLTP activity in the media. ApoA-I also increased phospholipid transfer activity of PLTP isolated from human plasma. This effect was dependent on apoA-I concentration but independent on apoA-I lipidation status. ApoE, ApoA-II and apoA-IV, but not immunoglobulins or bovine serum albumin, also increased PLTP activity. We also report that apoA-I protects PLTP from heat inactivation. Conclusion apoA-I enhances the phospholipid transfer activity of PLTP secreted from macrophage foam cells without affecting the PLTP mass. |
| اللغة: | English |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0e385a32f6f17154fda508827d261f43 http://hdl.handle.net/10138/162280 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....0e385a32f6f17154fda508827d261f43 |
| قاعدة البيانات: | OpenAIRE |
| الوصف غير متاح. |