Effects of low-level laser therapy and adipose-derived stem cells on the viability of autologous fat grafts: a preliminary study
| العنوان: | Effects of low-level laser therapy and adipose-derived stem cells on the viability of autologous fat grafts: a preliminary study |
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| المؤلفون: | Kyu Sang Lee, Byung Ho Shin, Young Soo Yoon, Sun Young Nam, Seunghee Lee, Chan Yeong Heo, Miji Lee, Kyung Hee Min |
| المصدر: | Archives of Aesthetic Plastic Surgery, Vol 27, Iss 3, Pp 81-87 (2021) |
| بيانات النشر: | Korean Society for Aesthetic Plastic Surgery, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | Pathology, medicine.medical_specialty, RD1-811, business.industry, medicine.medical_treatment, graft survival, Adipose tissue, General Medicine, gallium-aluminum-arsenide lasers, Gallium Aluminum Arsenide Lasers, low-level laser therapy, Autologous fat, stem cells, medicine, Surgery, Graft survival, Stem cell, business, Low level laser therapy |
| الوصف: | Background Autologous fat grafts are commonly used in cosmetic and reconstructive surgery, and various methods are used to improve their viability. Low-level laser therapy (LLLT) can enhance the proliferation, growth, and differentiation of various cell lines, including stem cells. Our study investigated and compared the effects of LLLT and the addition of adipose-derived stem cells (ADSCs) on the viability of fat grafts.Methods Twenty nude mice were divided into four groups: control (group 1), LLLT irradiation (group 2), ADSC addition (group 3), and LLLT irradiation+ADSC addition (group 4). ADSCs were combined with the fat tissue. LLLT irradiation was performed once daily for 1 week from the day of grafting. After 8 weeks, the weight, volume, histology, and Western blot findings of the grafted fat tissues were evaluated.Results The retention rate and volume of the fat tissue in groups 2, 3, and 4 were higher than that of group 1, but the difference was not statistically significant. The number of capillaries, histological parameters, and immunofluorescence staining analyses for CD68, CD31, fibroblast growth factor, and vascular endothelial growth factor (VEGF) showed no significant differences among the four groups. The expression level of VEGF was higher in group 2 than in the other groups, but not to a statistically significant level.Conclusions LLLT and ADSCs did not significantly improve the viability of autologous fat grafts. Therefore, further study is necessary to develop safe and effective methods to improve the viability of these grafts for clinical application. |
| تدمد: | 2288-9337 2234-0831 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0ec6d5bbf637a0578aefe4c9873cbf0b https://doi.org/10.14730/aaps.2021.00080 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....0ec6d5bbf637a0578aefe4c9873cbf0b |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 22889337 22340831 |
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