Suppression of Hepatocellular Carcinoma Progression through FOXM1 and EMT Inhibition via Hydroxygenkwanin-Induced miR-320a Expression
| العنوان: | Suppression of Hepatocellular Carcinoma Progression through FOXM1 and EMT Inhibition via Hydroxygenkwanin-Induced miR-320a Expression |
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| المؤلفون: | Wan-Hua Yang, Miaw-Jene Liou, Yann-Lii Leu, Chi-Yuan Chen, Junn-Liang Chang, Tong-Hong Wang, Chin-Chuan Chen, Li-Fang Chou |
| المصدر: | Biomolecules Volume 10 Issue 1 |
| بيانات النشر: | MDPI AG, 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | Male, 0301 basic medicine, Cell signaling, Carcinoma, Hepatocellular, Epithelial-Mesenchymal Transition, Mice, Nude, Biology, epithelial–mesenchymal transition, Southeast asian, complex mixtures, Biochemistry, Article, liver cancer, 03 medical and health sciences, 0302 clinical medicine, microRNA, medicine, Animals, Humans, Epithelial–mesenchymal transition, Molecular Biology, hydroxygenkwanin, Flavonoids, Cell growth, Forkhead Box Protein M1, Liver Neoplasms, FOXM1, Cancer, medicine.disease, miR-320a, Gene Expression Regulation, Neoplastic, MicroRNAs, 030104 developmental biology, 030220 oncology & carcinogenesis, Cancer research, Daphne, Liver cancer, Drugs, Chinese Herbal |
| الوصف: | Daphne genkwa, a Chinese medicinal herb, is used frequently in Southeast Asian countries to treat diseases the flavonoid hydroxygenkwanin (HGK) is extracted from its flower buds. The bioactivity of HGK, particularly as an anti-liver cancer agent, has not been explored. In this study, human hepatocellular carcinoma (HCC) cell lines and an animal xenograft model were employed to investigate both the activity of HGK against liver cancer and its cellular signaling mechanisms. HCC cells treated with HGK were subjected to cell function assays. Whole transcriptome sequencing was used to identify genes whose expression was influenced by HGK, and the flavonoid&rsquo s cancer suppression mechanisms were further investigated through gain- and loss-of-function assays. Finally, in vitro findings were tested in a mouse xenograft model. The data showed that HGK induced the expression of the microRNA miR-320a, which in turn inhibited the expression of the transcription factor &lsquo forkhead box protein M1&rsquo (FOXM1) and downstream FOXM1-regulated proteins related to epithelial&ndash mesenchymal transition, thereby leading to the suppression of liver cancer cell growth and invasion. Significant inhibition of tumor growth was also observed in HGK-treated mice. Hence, the present study demonstrated the activity of HGK against liver cancer and validated its potential use as a therapeutic agent. |
| وصف الملف: | application/pdf |
| تدمد: | 2218-273X |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0f176aff1a6296c558778193e90b595f https://doi.org/10.3390/biom10010020 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....0f176aff1a6296c558778193e90b595f |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 2218273X |
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