Phage gene expression and host responses lead to infection-dependent costs of CRISPR immunity
| العنوان: | Phage gene expression and host responses lead to infection-dependent costs of CRISPR immunity |
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| المؤلفون: | Ellinor O. Alseth, Loris Capria, Sean Meaden, Ambarish Biswas, Luca Lenzi, Stineke van Houte, Edze R. Westra, Sylvain Gandon |
| المساهمون: | Centre d’Ecologie Fonctionnelle et Evolutive (CEFE), Université Paul-Valéry - Montpellier 3 (UPVM)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-École pratique des hautes études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud])-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE) |
| المصدر: | The ISME Journal ISME J ISME Journal ISME Journal, Nature Publishing Group, 2020, ⟨10.1038/s41396-020-00794-w⟩ |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | Population, Gene Expression, Biology, Microbiology, Genome, Article, Microbial ecology, 03 medical and health sciences, Immune system, Immunity, Immunopathology, Gene expression, CRISPR, Bacteriophages, Clustered Regularly Interspaced Short Palindromic Repeats, education, Gene, Ecology, Evolution, Behavior and Systematics, ComputingMilieux_MISCELLANEOUS, 030304 developmental biology, Genetics, 0303 health sciences, education.field_of_study, 030306 microbiology, Correction, 3. Good health, Pseudomonas aeruginosa, [SDE.BE]Environmental Sciences/Biodiversity and Ecology, CRISPR-Cas Systems |
| الوصف: | CRISPR-Cas immune systems are widespread in bacteria and archaea, but not ubiquitous. Previous work has demonstrated that CRISPR immunity is associated with an infection-induced fitness cost, which may help explain the patchy distribution observed. However, the mechanistic basis of this cost has remained unclear. Using Pseudomonas aeruginosa PA14 and its phage DMS3vir as a model, we perform a 30-day evolution experiment under phage mediated selection. We demonstrate that although CRISPR is initially selected for, bacteria carrying mutations in the phage receptor rapidly invade the population following subsequent reinfections. We then test three potential mechanisms for the observed cost of CRISPR: (1) autoimmunity from the acquisition of self-targeting spacers, (2) immunopathology or energetic costs from increased cas gene expression and (3) toxicity caused by phage gene expression prior to CRISPR-mediated cleavage. We find that phages can express genes before the immune system clears the infection and that expression of these genes can have a negative effect on host fitness. While infection does not lead to increased expression of cas genes, it does cause differential expression of multiple other host processes that may further contribute to the cost of CRISPR immunity. In contrast, we found little support for infection-induced autoimmunological and immunopathological effects. Phage gene expression prior to cleavage of the genome by the CRISPR-Cas immune system is therefore the most parsimonious explanation for the observed phage-induced fitness cost. |
| تدمد: | 1751-7362 1751-7370 |
| DOI: | 10.1038/s41396-020-00794-w |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0f706d3621ac8afa1a852b8e040a100c |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....0f706d3621ac8afa1a852b8e040a100c |
| قاعدة البيانات: | OpenAIRE |
Find this article in full text from Springer Verlag. | تدمد: | 17517362 17517370 |
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| DOI: | 10.1038/s41396-020-00794-w |