Regulated dicing of pre-mir-144 via reshaping of its terminal loop
| العنوان: | Regulated dicing of pre-mir-144 via reshaping of its terminal loop |
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| المؤلفون: | Renfu Shang, Dmitry A Kretov, Scott I Adamson, Thomas Treiber, Nora Treiber, Jeffrey Vedanayagam, Jeffrey H Chuang, Gunter Meister, Daniel Cifuentes, Eric C Lai |
| المصدر: | Nucleic Acids Research. 50:7637-7654 |
| بيانات النشر: | Oxford University Press (OUP), 2022. |
| سنة النشر: | 2022 |
| مصطلحات موضوعية: | Ribonuclease III, MicroRNAs, Genetics, Animals, Humans, Zebrafish |
| الوصف: | Although the route to generate microRNAs (miRNAs) is often depicted as a linear series of sequential and constitutive cleavages, we now appreciate multiple alternative pathways as well as diverse strategies to modulate their processing and function. Here, we identify an unusually profound regulatory role of conserved loop sequences in vertebrate pre-mir-144, which are essential for its cleavage by the Dicer RNase III enzyme in human and zebrafish models. Our data indicate that pre-mir-144 dicing is positively regulated via its terminal loop, and involves the ILF3 complex (NF90 and its partner NF45/ILF2). We provide further evidence that this regulatory switch involves reshaping of the pre-mir-144 apical loop into a structure that is appropriate for Dicer cleavage. In light of our recent findings that mir-144 promotes the nuclear biogenesis of its neighbor mir-451, these data extend the complex hierarchy of nuclear and cytoplasmic regulatory events that can control the maturation of clustered miRNAs. |
| تدمد: | 1362-4962 0305-1048 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0f7d475461f8b0c6d2e33ded1436afb4 https://doi.org/10.1093/nar/gkac568 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....0f7d475461f8b0c6d2e33ded1436afb4 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 13624962 03051048 |
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