Activity of Lac repressor anchored to the Escherichia coli inner membrane
| العنوان: | Activity of Lac repressor anchored to the Escherichia coli inner membrane |
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| المؤلفون: | Bodo Rak, Jana Reinhardt, Boris Görke |
| المصدر: | Nucleic Acids Research |
| بيانات النشر: | Oxford University Press, 2005. |
| سنة النشر: | 2005 |
| مصطلحات موضوعية: | Operator Regions, Genetic, Transcription, Genetic, Recombinant Fusion Proteins, Repressor, Lac repressor, Biology, medicine.disease_cause, Article, 03 medical and health sciences, Bacterial Proteins, Genetics, medicine, Escherichia coli, Lac Repressors, Inner membrane, 030304 developmental biology, Regulation of gene expression, 0303 health sciences, Escherichia coli Proteins, 030302 biochemistry & molecular biology, Membrane Proteins, Gene Expression Regulation, Bacterial, Chromosomes, Bacterial, Fusion protein, Cell biology, Repressor Proteins, nucleic acids, Kinetics, Biochemistry, Membrane protein, Cytoplasm |
| الوصف: | The transient inactivation of gene regulatory proteins by their sequestration to the cytoplasmic membrane in response to cognate signals is an increasingly recognized mechanism of gene regulation in bacteria. It remained to be shown, however, whether tethering to the membrane per se could be responsible for inactivation, i.e. whether such relocation leads to a spatial separation from the chromosome that results in inactivity or whether other mechanisms are involved. We, therefore, investigated the activity of Lac repressor artificially attached to the Escherichia coli cytoplasmic membrane. We demonstrate that this chimeric protein perfectly represses transcription initiated at the tac operator–promoter present on a plasmid and even in the chromosome. Moreover, this repression is inducible as normal. The data suggest that proteins localized to the inner face of the cytoplasmic membrane in principle have unrestricted access to the chromosome. Thus sequestration to the membrane in terms of physical separation from the chromosome cannot account alone for the inactivation of regulatory proteins. Other mechanisms, like induction of a conformational change or masking of binding domains are required additionally. peerReviewed |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 1362-4962 0305-1048 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0fb4a453c49d94788ede07636f5daca6 http://europepmc.org/articles/PMC1088070 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....0fb4a453c49d94788ede07636f5daca6 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 13624962 03051048 |
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