Olfactory Discrimination Training Up-Regulates and Reorganizes Expression of MicroRNAs in Adult Mouse Hippocampus
| العنوان: | Olfactory Discrimination Training Up-Regulates and Reorganizes Expression of MicroRNAs in Adult Mouse Hippocampus |
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| المؤلفون: | Giovanni Lugli, John M. Davis, Neil R. Smalheiser, Angela L Lenon, Vetle I. Torvik, John Larson |
| المصدر: | ASN Neuro, Vol 2 (2010) ASN NEURO |
| بيانات النشر: | SAGE Publications, 2010. |
| سنة النشر: | 2010 |
| مصطلحات موضوعية: | Male, Hippocampus, TOP, terminal oligopyrimidine, Mice, Random Allocation, Discrimination, Psychological, 0302 clinical medicine, LTP, long-term potentiation, Neurotrophic factors, miRNA, microRNA, RISC, RNA-induced silencing complex, TLDA, TaqMan® Low Density Arrays, 0303 health sciences, microRNA, Behavior, Animal, biology, General Neuroscience, DHPG, (S)-3,5-dihydroxyphenylglycine, NMDA, N-methyl-d-aspartate, Up-Regulation, Smell, pre-miR, miRNA small hairpin precursor, olfactory discrimination, BDNF, brain-derived neurotrophic factor, RT-PCR, reverse transcription-PCR, CREB1, Psychology, S1, Ct, threshold cycle value, S9, MEF2, myocyte enhancer factor-2, lcsh:RC321-571, 03 medical and health sciences, Downregulation and upregulation, snoRNA, small nucleolar RNA, In vivo, Research article, Animals, Learning, Protein kinase A, lcsh:Neurosciences. Biological psychiatry. Neuropsychiatry, 030304 developmental biology, dicer, synaptic plasticity, pri-miR, primary miRNA gene transcript, Mice, Inbred C57BL, MicroRNAs, Synaptic plasticity, biology.protein, Neurology (clinical), Neuroscience, 030217 neurology & neurosurgery, Dicer |
| الوصف: | Adult male mice (strain C57Bl/6J) were trained to execute nose-poke responses for water reinforcement; then they were randomly assigned to either of two groups: Olfactory discrimination training (exposed to two odours with reward contingent upon correctly responding to one odour) or pseudo-training (exposed to two odours with reward not contingent upon response). These were run in yoked fashion and killed when the discrimination-trained mouse reached a learning criterion of 70% correct responses in 20 trials, occurring after three sessions (a total of ~40 min of training). The hippocampus was dissected bilaterally from each mouse ( N=7 in each group) and profiling of 585 miRNAs (microRNAs) was carried out using multiplex RT–PCR (reverse transcription–PCR) plates. A significant global up-regulation of miRNA expression was observed in the discrimination training versus pseudo-training comparison; when tested individually, 29 miRNAs achieved significance at P=0.05. miR-10a showed a 2.7-fold increase with training, and is predicted to target several learning-related mRNAs including BDNF (brain-derived neurotrophic factor), CAMK2b (calcium/calmodulin-dependent protein kinase IIβ), CREB1 (cAMP-response-element-binding protein 1) and ELAVL2 [ELAV (embryonic lethal, abnormal vision, Drosophila)-like; Hu B]. Analysis of miRNA pairwise correlations revealed the existence of several miRNA co-expression modules that were specific to the training group. These in vivo results indicate that significant, dynamic and co-ordinated changes in miRNA expression accompany early stages of learning. |
| تدمد: | 1759-0914 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0fd8f0025f9c601c41785570167fe75a https://doi.org/10.1042/an20090055 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....0fd8f0025f9c601c41785570167fe75a |
| قاعدة البيانات: | OpenAIRE |
PDF full text from Publisher | تدمد: | 17590914 |
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