In vitro binding of [14C]2,5-hexanedione to rat neuronal cytoskeletal proteins
| العنوان: | In vitro binding of [14C]2,5-hexanedione to rat neuronal cytoskeletal proteins |
|---|---|
| المؤلفون: | Mohamed B. Abou-Donia, Christine L. Lanning, Kenneth R. Wilmarth |
| المصدر: | Neurochemical Research. 19:1165-1173 |
| بيانات النشر: | Springer Science and Business Media LLC, 1994. |
| سنة النشر: | 1994 |
| مصطلحات موضوعية: | Male, Neurofilament, Immunoblotting, Biochemistry, Rats, Sprague-Dawley, Radioligand Assay, Cellular and Molecular Neuroscience, chemistry.chemical_compound, Neurofilament Proteins, Microtubule, medicine, Animals, Hexane-2,5-dione, Neurotoxin, Pyrroles, Carbon Radioisotopes, Sodium dodecyl sulfate, Axon, Cytoskeleton, Neurons, biology, General Medicine, Rats, Cytoskeletal Proteins, Hexanones, medicine.anatomical_structure, Tubulin, chemistry, Microtubule Proteins, Biophysics, biology.protein, Oxidation-Reduction |
| الوصف: | 2,5-Hexanedione (2,5-HD) induces central-peripheral axonpathy characterized by the accumulation of 10-nm neurofilaments proximal to the nodes of Ranvier and a Wallerian-type degeneration. It has been postulated that neurofilament crosslinking may be involved in the production of this axonopathy. A potential initiating event in this neurotoxic process may be the direct binding of 2,5-HD to neurofilament and microtubule proteins. In this study, the in vitro binding of [14C]2,5-HD to neurofilament and microtubule proteins was examined. Neurofilament proteins isolated from rat spinal cord or microtubule proteins isolated from rat brain were incubated in the presence of 2,5-HD at concentrations ranging from 25 to 500 mM. Quantitative analysis of sodium dodecyl sulfate (SDS) polyacrylamide gels revealed a dose- and time-dependent binding of 2,5-HD to both neurofilament proteins and microtubule proteins. Expressed as pmol 2,5-HD bound per microgram protein, the observed relative binding was MAP2NF160NF200NF68tubulin. These data demonstrate the direct binding of 2,5-HD to cytoskeletal proteins including both neurofilaments and microtubules. |
| تدمد: | 1573-6903 0364-3190 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0ffba779a21e842c0ee627b4240c179e https://doi.org/10.1007/bf00965151 |
| حقوق: | CLOSED |
| رقم الأكسشن: | edsair.doi.dedup.....0ffba779a21e842c0ee627b4240c179e |
| قاعدة البيانات: | OpenAIRE |
Find this article in full text from Springer Verlag. | تدمد: | 15736903 03643190 |
|---|