Toward a sensible single antigen bead cut-off based on kidney graft survival
| العنوان: | Toward a sensible single antigen bead cut-off based on kidney graft survival |
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| المؤلفون: | Bram W. Wisse, Johan W. de Fijter, Shaikh A. Nurmohamed, Karlijn A M I van der Pant, N M Lardy, Ineke J. M. ten Berge, M. Gelens, Sebastiaan Heidt, Michiel L. Bots, Andries J. Hoitsma, Adriaan C.A.D. Drop, Arnold van der Meer, Luuk B. Hilbrands, Frans J. van Ittersum, Frederike J. Bemelman, Dave L. Roelen, Paul J M van der Boog, Jan-Stephan F. Sanders, Arjan D. van Zuilen, Loes Plaisier, Wendy Swelsen, Michiel G. H. Betjes, Cornelis E. Hack, Franka E. van Reekum, Elly M. van Duijnhoven, Laura Bungener, Caroline Roozendaal, Henny G. Otten, Marije C. Baas, Bouke G. Hepkema, Neelke C. van der Weerd, Christina E.M. Voorter, Frans H.J. Claas, Eric Spierings, Lotte Wieten, Wil A. Allebes, Irma Joosten, Marianne C. Verhaar, Marcel G.J. Tilanus, Annechien J. A. Lambeck, Elena G. Kamburova, Maarten H. L. Christiaans, Marc A. Seelen |
| المساهمون: | Oral and Maxillofacial Surgery, Erasmus MC other, Internal Medicine, Groningen Institute for Organ Transplantation (GIOT), Groningen Kidney Center (GKC), Nephrology, ACS - Atherosclerosis & ischemic syndromes, ACS - Diabetes & metabolism, AII - Inflammatory diseases, Interne Geneeskunde, RS: GROW - R3 - Innovative Cancer Diagnostics & Therapy, MUMC+: DA TI Staf (9), MUMC+: DA TI Laboratorium (9), MUMC+: MA Nefrologie (9), APH - Aging & Later Life |
| المصدر: | Transplantation, 103(4), 789. Lippincott Williams and Wilkins Transplantation Transplantation, 103, 789-797 Transplantation, 103(4), 789-797. Lippincott Williams & Wilkins Transplantation, 103, 4, pp. 789-797 Transplantation, 103(4), 789-797. LIPPINCOTT WILLIAMS & WILKINS Transplantation, 103(4), 789-797 Transplantation, 103(4), 789-797. Lippincott Williams and Wilkins Wisse, B W, Kamburova, E G, Joosten, I, Allebes, W A, van der Meer, A, Hilbrands, L B, Baas, M C, Spierings, E, Hack, C E, van Reekum, F E, van Zuilen, A D, Verhaar, M C, Bots, M L, Drop, A C A D, Plaisier, L, Seelen, M A J, Stephan Sanders, J, Hepkema, B G, Lambeck, A J A, Bungener, L B, Roozendaal, C, Tilanus, M G J, Voorter, C E, Wieten, L, van Duijnhoven, E M, Gelens, M A C J, Christiaans, M H L, van Ittersum, F J, Nurmohamed, S A, Lardy, N M, Swelsen, W, van der Pant, K A M I, van der Weerd, N C, ten Berge, I J M, Bemelman, F J, Hoitsma, A J, van der Boog, P J M, de Fijter, J W, Betjes, M G H, Heidt, S, Roelen, D L, Claas, F H & Otten, H G 2019, ' Toward a Sensible Single-antigen Bead Cutoff Based on Kidney Graft Survival ', Transplantation, vol. 103, no. 4, pp. 789-797 . https://doi.org/10.1097/TP.0000000000002357 |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | medicine.medical_specialty, Urology, Other Research Radboud Institute for Molecular Life Sciences [Radboudumc 0], Human leukocyte antigen, 030230 surgery, Fluorescence, 03 medical and health sciences, 0302 clinical medicine, HLA Antigens, Isoantibodies, STRENGTH, Medicine, Cutoff, Humans, Single antigen bead, Kidney transplantation, Kidney, Transplantation, business.industry, Graft Survival, Original Clinical Science—General, medicine.disease, Kidney Transplantation, Tissue Donors, body regions, HLA, medicine.anatomical_structure, Risk stratification, ANTIBODIES, ComputingMethodologies_DOCUMENTANDTEXTPROCESSING, 030211 gastroenterology & hepatology, Graft survival, Renal disorders Radboud Institute for Health Sciences [Radboudumc 11], business, Inflammatory diseases Radboud Institute for Molecular Life Sciences [Radboudumc 5] |
| الوصف: | Supplemental Digital Content is available in the text. Background. There is no consensus in the literature on the interpretation of single-antigen bead positive for a specific HLA antibody. Methods. To inform the debate, we studied the relationship between various single-antigen bead positivity algorithms and the impact of resulting donor-specific HLA antibody (DSA) positivity on long-term kidney graft survival in 3237 deceased-donor transplants. Results. First, we showed that the interassay variability can be greatly reduced when working with signal-to-background ratios instead of absolute median fluorescence intensities (MFIs). Next, we determined pretransplant DSA using various MFI cutoffs, signal-to-background ratios, and combinations thereof. The impact of the various cutoffs was studied by comparing the graft survival between the DSA-positive and DSA-negative groups. We did not observe a strong impact of various cutoff levels on 10-year graft survival. A stronger relationship between the cutoff level and 1-year graft survival for DSA-positive transplants was found when using signal-to-background ratios, most pronounced for the bead of the same HLA locus with lowest MFI taken as background. Conclusions. With respect to pretransplant risk stratification, we propose a signal-to-background ratio-6 (using the bead of the same HLA-locus with lowest MFI as background) cutoff of 15 combined with an MFI cutoff of 500, resulting in 8% and 21% lower 1- and 10-year graft survivals, respectively, for 8% DSA-positive transplants. |
| وصف الملف: | text/plain; application/pdf; image/pdf |
| اللغة: | English |
| تدمد: | 0041-1337 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::10d430eee2ec0ef17a10647b022ac4fd https://dspace.library.uu.nl/handle/1874/391348 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....10d430eee2ec0ef17a10647b022ac4fd |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 00411337 |
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