Formaldehyde and Glutaraldehyde Inactivation of Bacterial Tier 1 Select Agents in Tissues
| العنوان: | Formaldehyde and Glutaraldehyde Inactivation of Bacterial Tier 1 Select Agents in Tissues |
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| المؤلفون: | Arthur M. Friedlander, Jared M. Crumpler, Melissa G. Hunter, Christopher P. Klimko, David E. Harbourt, Christopher K. Cote, Avery V. Quirk, Steven A. Tobery, Jennifer Chua, Jennifer L. Shoe, Donald J. Chabot, Christopher L. Jensen, Joel A. Bozue, Sara I. Ruiz |
| المصدر: | Emerging Infectious Diseases, Vol 25, Iss 5, Pp 919-926 (2019) |
| بيانات النشر: | Centers for Disease Control and Prevention (CDC), 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | Microbiology (medical), Burkholderia pseudomallei, Time Factors, Yersinia pestis, Epidemiology, Guinea Pigs, 030231 tropical medicine, lcsh:Medicine, Virulence, Burkholderia mallei, lcsh:Infectious and parasitic diseases, Microbiology, 03 medical and health sciences, chemistry.chemical_compound, 0302 clinical medicine, Formaldehyde, Animals, lcsh:RC109-216, 030212 general & internal medicine, Francisella tularensis, Spores, Bacterial, Microbial Viability, Bacteria, biology, Research, lcsh:R, biology.organism_classification, Bacillus anthracis, Infectious Diseases, chemistry, Glutaral, Organ Specificity, Tier 1, Glutaraldehyde, Disinfectants |
| الوصف: | For safety, designated Select Agents in tissues must be inactivated and viability tested before the tissue undergoes further processing and analysis. In response to the shipping of samples of “inactivated” Bacillus anthracis that inadvertently contained live spores to nonregulated entities and partners worldwide, the Federal Register now mandates in-house validation of inactivation procedures and standardization of viability testing to detect live organisms in samples containing Select Agents that have undergone an inactivation process. We tested and validated formaldehyde and glutaraldehyde inactivation procedures for animal tissues infected with virulent B. anthracis, Burkholderia pseudomallei, Francisella tularensis, and Yersinia pestis. We confirmed that our fixation procedures for tissues containing these Tier 1 Select Agents resulted in complete inactivation and that our validated viability testing methods do not interfere with detection of live organisms. Institutions may use this work as a guide to develop and conduct their own testing to comply with the policy. |
| تدمد: | 1080-6059 1080-6040 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::10e77321ca5c0032c3e100969ef651ca https://doi.org/10.3201/eid2505.180928 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....10e77321ca5c0032c3e100969ef651ca |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 10806059 10806040 |
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