Generation and Transcriptome Profiling of Slr1-d7 and Slr1-d8 Mutant Lines with a New Semi-Dominant Dwarf Allele of SLR1 Using the CRISPR/Cas9 System in Rice
| العنوان: | Generation and Transcriptome Profiling of Slr1-d7 and Slr1-d8 Mutant Lines with a New Semi-Dominant Dwarf Allele of SLR1 Using the CRISPR/Cas9 System in Rice |
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| المؤلفون: | Jihyeon Yu, Yong-Gu Cho, Kwon Kyoo Kang, Sangsu Bae, Dong Hyun Kim, Hyo Ju Lee, Jong Hee Kim, Yu Jin Jung |
| المصدر: | International Journal of Molecular Sciences Volume 21 Issue 15 International Journal of Molecular Sciences, Vol 21, Iss 5492, p 5492 (2020) |
| بيانات النشر: | MDPI, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | 0106 biological sciences, 0301 basic medicine, Mutant, Genetically modified crops, medicine.disease_cause, 01 natural sciences, lcsh:Chemistry, Plant Growth Regulators, Gene Expression Regulation, Plant, Gene expression, CRISPR, lcsh:QH301-705.5, Spectroscopy, Plant Proteins, Genetics, Mutation, GA, food and beverages, General Medicine, Plants, Genetically Modified, GA signaling, Computer Science Applications, Phenotype, Dwarf, Signal Transduction, GA20OX2, DELLA/TVHYNP, Biology, Catalysis, Deep sequencing, Article, Inorganic Chemistry, 03 medical and health sciences, medicine, Amino Acid Sequence, Physical and Theoretical Chemistry, Molecular Biology, Gene, CRISPR/Cas9, Alleles, Cas9, Gene Expression Profiling, Organic Chemistry, Oryza, Gibberellins, 030104 developmental biology, lcsh:Biology (General), lcsh:QD1-999, CRISPR-Cas Systems, Transcriptome, 010606 plant biology & botany |
| الوصف: | The rice SLR1 gene encodes the DELLA protein, and a loss-of-function mutation is dwarfed by inhibiting plant growth. We generate slr1-d mutants with a semi-dominant dwarf phenotype to target mutations of the DELLA/TVHYNP domain using CRISPR/Cas9 genome editing in rice. Sixteen genetic edited lines out of 31 transgenic plants were generated. Deep sequencing results showed that the mutants had six different mutation types at the target site of the TVHYNP domain of the SLR1 gene. The homo-edited plants selected individuals without DNA (T-DNA) transcribed by segregation in the T1 generation. The slr1-d7 and slr1-d8 plants caused a gibberellin (GA)-insensitive dwarf phenotype with shrunken leaves and shortened internodes. A genome-wide gene expression analysis by RNA-seq indicated that the expression levels of two GA-related genes, GA20OX2 (Gibberellin oxidase) and GA3OX2, were increased in the edited mutant plants, suggesting that GA20OX2 acts as a convert of GA12 signaling. These mutant plants are required by altering GA responses, at least partially by a defect in the phytohormone signaling system process and prevented cell elongation. The new mutants, namely, the slr1-d7 and slr1-d8 lines, are valuable semi-dominant dwarf alleles with potential application value for molecule breeding using the CRISPR/Cas9 system in rice. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 1422-0067 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::11222765ec654d7800a38a74765bf01a http://europepmc.org/articles/PMC7432230 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....11222765ec654d7800a38a74765bf01a |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14220067 |
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