Serum-based microRNA signatures in early diagnosis and prognosis prediction of colon cancer
| العنوان: | Serum-based microRNA signatures in early diagnosis and prognosis prediction of colon cancer |
|---|---|
| المؤلفون: | Vladimír Procházka, Petra Vychytilova-Faltejskova, Katerina Slaba, Zdenek Kala, Zdenka Kosarova, Pavel Fabian, Lenka Radová, Marek Svoboda, Milana Šachlová, Rostislav Vyzula, Tomáš Grolich, Ondrej Slaby, Igor Kiss |
| المصدر: | Carcinogenesis. 37:941-950 |
| بيانات النشر: | Oxford University Press (OUP), 2016. |
| سنة النشر: | 2016 |
| مصطلحات موضوعية: | Male, 0301 basic medicine, Oncology, Cancer Research, medicine.medical_specialty, Colorectal cancer, Disease, 03 medical and health sciences, 0302 clinical medicine, Blood serum, Internal medicine, microRNA, Biomarkers, Tumor, medicine, Humans, Stage (cooking), Early Detection of Cancer, Aged, Neoplasm Staging, business.industry, General Medicine, Middle Aged, Prognosis, medicine.disease, 3. Good health, Gene Expression Regulation, Neoplastic, Gene expression profiling, MicroRNAs, Circulating MicroRNA, 030104 developmental biology, 030220 oncology & carcinogenesis, Colonic Neoplasms, Biomarker (medicine), Female, business |
| الوصف: | Early detection of colorectal cancer is the main prerequisite for successful treatment and reduction of mortality. Circulating microRNAs were previously identified as promising diagnostic, prognostic and predictive biomarkers. The purpose of this study was to identify serum microRNAs enabling early diagnosis and prognosis prediction of colon cancer. In total, serum samples from 427 colon cancer patients and 276 healthy donors were included in three-phase biomarker study. Large-scale microRNA expression profiling was performed using Illumina small RNA sequencing. Diagnostic and prognostic potential of identified microRNAs was validated on independent training and validation sets of samples using RT-qPCR. Fifty-four microRNAs were found to be significantly deregulated in serum of colon cancer patients compared to healthy donors (P < 0.01). A diagnostic four-microRNA signature consisting of miR-23a-3p, miR-27a-3p, miR-142-5p and miR-376c-3p was established (AUC = 0.917), distinguishing colon cancer patients from healthy donors with sensitivity of 89% and specificity of 81% (AUC = 0.922). This panel of microRNAs exhibited high diagnostic performance also when analyzed separately in colon cancer patients in early stages of the disease (T1-4N0M0; AUC = 0.877). Further, a prognostic panel based on the expression of miR-23a-3p and miR-376c-3p independent of TNM stage was established (HR 2.30; 95% CI 1.44-3.66; P < 0.0004). In summary, highly sensitive signatures of circulating microRNAs enabling non-invasive early detection and prognosis prediction of colon cancer were identified. |
| تدمد: | 1460-2180 0143-3334 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::11267b31f148a77060c190ca9056acec https://doi.org/10.1093/carcin/bgw078 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....11267b31f148a77060c190ca9056acec |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14602180 01433334 |
|---|