Solitary restriction endonucleases in prokaryotic genomes
| العنوان: | Solitary restriction endonucleases in prokaryotic genomes |
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| المؤلفون: | Andrei V. Alexeevski, Sergey A. Spirin, A. M. Lyashchuk, Anna S. Ershova, Mikhail Vasiliev, Anna S. Karyagina, Vladimir G. Lunin |
| المصدر: | Nucleic Acids Research |
| بيانات النشر: | Oxford University Press, 2012. |
| سنة النشر: | 2012 |
| مصطلحات موضوعية: | Genetics, Methyltransferase, Deoxyribonucleases, Type I Site-Specific, Computational Biology, Genomics, DNA Restriction Enzymes, Biology, Genome, Restriction enzyme, chemistry.chemical_compound, Rebase, chemistry, Genome, Archaeal, DNA methylation, biology.protein, Deoxyribonucleases, Type II Site-Specific, Gene, DNA Modification Methylases, DNA, Genome, Bacterial |
| الوصف: | Prokaryotic restriction-modification (R-M) systems defend the host cell from the invasion of a foreign DNA. They comprise two enzymatic activities: specific DNA cleavage activity and DNA methylation activity preventing cleavage. Typically, these activities are provided by two separate enzymes: a DNA methyltransferase (MTase) and a restriction endonuclease (RE). In the absence of a corresponding MTase, an RE of Type II R-M system is highly toxic for the cell. Genes of the R-M system are linked in the genome in the vast majority of annotated cases. There are only a few reported cases in which the genes of MTase and RE from one R-M system are not linked. Nevertheless, a few hundreds solitary RE genes are present in the Restriction Enzyme Database (http://rebase.neb.com) annotations. Using the comparative genomic approach, we analysed 272 solitary RE genes. For 57 solitary RE genes we predicted corresponding MTase genes located distantly in a genome. Of the 272 solitary RE genes, 99 are likely to be fragments of RE genes. Various explanations for the existence of the remaining 116 solitary RE genes are also discussed. |
| اللغة: | English |
| تدمد: | 1362-4962 0305-1048 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1139ab189e421cbf2c5b24331629203b http://europepmc.org/articles/PMC3488263 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....1139ab189e421cbf2c5b24331629203b |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 13624962 03051048 |
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