Prevalence and genomic analysis of ESBL-producingEscherichia coliin retail raw meats in Singapore
| العنوان: | Prevalence and genomic analysis of ESBL-producingEscherichia coliin retail raw meats in Singapore |
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| المؤلفون: | Joergen Schlundt, Frank Møller Aarestrup, Moon Y. F. Tay, Yang Zhong, Lee Ching Ng, Kyaw Thu Aung, Siyao Guo, Kelyn Lee Ghee Seow, Pimlapas Leekitcharoenphon |
| المصدر: | Journal of Antimicrobial Chemotherapy. 76:601-605 |
| بيانات النشر: | Oxford University Press (OUP), 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | Microbiology (medical), Veterinary medicine, Meat, food.ingredient, Esbl production, Food Contamination, Biology, medicine.disease_cause, beta-Lactamases, Colistin resistance, food, Drug Resistance, Bacterial, Escherichia coli, Prevalence, medicine, Animals, Agar, Pharmacology (medical), Raw meat, Pharmacology, Singapore, business.industry, Escherichia coli Proteins, Aminoglycoside resistance, Genomics, Antimicrobial, Food safety, Anti-Bacterial Agents, Infectious Diseases, Cattle, business, Chickens |
| الوصف: | ObjectivesTo determine the prevalence and genetic characteristics of ESBL-producing Escherichia coli in retail raw meats from Singapore markets.MethodsA total of 634 raw meat (chicken, pork and beef) samples were collected from markets in Singapore during June 2017–October 2018. The samples were enriched overnight and then incubated on Brilliance™ ESBL Agar. Presumptive ESBL isolates were confirmed using the double-disc synergy test. Confirmed ESBL-producing E. coli were sent for WGS and bioinformatic analysis was performed.ResultsThe prevalence of ESBL-producing E. coli in chicken, pork and beef meats was 51.2% (109/213), 26.9% (58/216) and 7.3% (15/205), respectively. A total of 225 ESBL-producing E. coli were isolated from 184 samples. β-Lactam resistance genes were detected in all isolates. After β-lactam resistance genes, the most common antimicrobial resistance genes detected were aminoglycoside resistance genes (92.4%). One hundred and seventy-two (76.4%), 102 (45.3%) and 52 (23.1%) isolates carried blaCTX-M genes, blaTEM genes and blaSHV genes, respectively. blaCTX-M-55 (57/225, 25.3%) and blaCTX-M-65 (40/225, 17.8%) were the most frequent ESBL genes. Colistin resistance genes (including mcr-1, mcr-3 and mcr-5) were found in 15.6% of all isolates.ConclusionsThis study indicates that ESBL-producing E. coli are widely found in retail raw meats, especially chicken, in Singapore. Occurrence of MDR (resistance to at least three classes of antimicrobial) and colistin resistance genes in retail raw meat suggests potential food safety and public health risks. |
| تدمد: | 1460-2091 0305-7453 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::11578660976a7723ad747f28cff2b07a https://doi.org/10.1093/jac/dkaa461 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....11578660976a7723ad747f28cff2b07a |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14602091 03057453 |
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