Effect of Hepatic Differentiation on Fatty Acid Composition of Induced Pluripotent Stem Cells Derived from Human Dermal Fibroblasts
| العنوان: | Effect of Hepatic Differentiation on Fatty Acid Composition of Induced Pluripotent Stem Cells Derived from Human Dermal Fibroblasts |
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| المؤلفون: | Yagoub Rahimi, Maghsod Shaaker, Mohammad Nouri, Hojjatollah Nozad Charoudeh, Nasim Parsafam, Masoud Darabi, Amir Mehdizadeh |
| المصدر: | İstanbul Medical Journal, Vol 19, Iss 2, Pp 113-118 (2018) |
| بيانات النشر: | Galenos Yayinevi, 2018. |
| سنة النشر: | 2018 |
| مصطلحات موضوعية: | Adult stem cells, lipids, business.industry, lcsh:R, lcsh:Medicine, Medicine, Fatty acid composition, endoderm, business, Induced pluripotent stem cell, cell lineage, Cell biology |
| الوصف: | Introduction: Human induced pluripotent stem cells (hiPSCs) have been recognized as key progenitor cells for liver regeneration, which could potentially be used in the repair of liver injury. Differentiation of stem cells involves specific changes in lipid metabolism to form competent hepatic cells. However, the relationship between the hepatic differentiation of hiPSCs and change in cellular lipids has not been well characterized. The aim of the present study was to determine the fatty acid pattern during hepatic differentiation of hiPSCs derived from dermal fibroblasts.Methods: Hepatocyte differentiation was induced in three stages using Wnt family member 3A, hepatocyte growth factor, and oncostatin M and was impaired by a chemical extracellular signal-regulated kinase signaling inhibitor. Hepatocyte-specific metabolic markers including gamma-glutamyltransferase and aminotransferases were evaluated using kinetic chromogenic assay kits. During the hepatic differentiation of hiPSCs, changes in individual fatty acids were determined at multiple time points using gas-liquid chromatography.Results: Significant increases were observed in hepatic enzyme markers during hepatic differentiation. Endodermal induction produced a transient increase in saturated fatty acids (33%, p |
| تدمد: | 2148-094X 2619-9793 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::11b7822340d1259d32a9dc86d2656ae9 https://doi.org/10.5152/imj.2018.60465 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....11b7822340d1259d32a9dc86d2656ae9 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 2148094X 26199793 |
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