Pyrrolidinedithiocarbamic Acid Ammonium Salt Inhibits Apop-tosis and Phenotypic Transformation of Co-Culture of Myeloma Cells and Renal Tubular Epithelial Cells by Reducing the Secretion of Light Chain Protein
| العنوان: | Pyrrolidinedithiocarbamic Acid Ammonium Salt Inhibits Apop-tosis and Phenotypic Transformation of Co-Culture of Myeloma Cells and Renal Tubular Epithelial Cells by Reducing the Secretion of Light Chain Protein |
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| المؤلفون: | Jie Bao, Yuefei Wang, Ling Li, Xinyu Cui, Fengxia Du, Lili Bi, Xiaoyan Yu, Jun Sun |
| المصدر: | Iranian Journal of Public Health, Vol 49, Iss 11, Pp 2078-2086 (2020) Iranian Journal of Public Health |
| بيانات النشر: | Tehran University of Medical Sciences, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | myeloma nephropathy, Cell, Salt (chemistry), Immunoglobulin light chain, 03 medical and health sciences, chemistry.chemical_compound, 0302 clinical medicine, renal tubular epithelial cells, medicine, Ammonium, Secretion, 030212 general & internal medicine, chemistry.chemical_classification, 030505 public health, lcsh:Public aspects of medicine, Public Health, Environmental and Occupational Health, light chain protein, lcsh:RA1-1270, Molecular biology, Phenotype, Transformation (genetics), medicine.anatomical_structure, chemistry, Apoptosis, Original Article, 0305 other medical science |
| الوصف: | Background: We investigate the effects of NFκB inhibitor pyrrolidinedithiocarbamic acid ammonium salt (PDTC) on the viability, apoptosis and cell phenotype of HK-2 cells in the co-culture system of myeloma cells in renal tubular epithelial cells. Methods: This study was performed in Qiqihar Medical University, Qiqihar, China from Jun 2018 to Jan 2019. RPMI-8226 cells and HK-2 cells were inoculated in the co-culture chamber and cultured to establish the co-culture system. An immunoturbidimetric assay was performed to detect κ light chain and λ light chain in RPMI-8226 cells. The effect of PDTC on the secretion of κ light chain and λ light chain of RPMI-8226 cells was detected by immunoturbidimetry and the ratio was calculated. Results: PDTC significantly increased the viability of HK-2 cells. PDTC reduced the apoptosis of renal tubular epithelial cells. After PDTC treatment, the expression of cell surface marker E-cadherin decreased, and the expression of α-SMA increased, which induced the renal interstitial fibrosis. The secretion of κ light chain and λ light chain of RPMI-8226 cells was significantly decreased after the addition of PDTC, but the ratio was not changed. Conclusion: PDTC can inhibit the cell activity, promote apoptosis, and reduce the secretion of secretion of κ light chain and λ light chain through inhibiting the NF-κB pathway activation of myeloma cell RPMI-8226. |
| اللغة: | English |
| تدمد: | 2251-6093 2251-6085 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::11df0249d51b7cdff890e5de5144895b https://ijph.tums.ac.ir/index.php/ijph/article/view/20484 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....11df0249d51b7cdff890e5de5144895b |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 22516093 22516085 |
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