High-resolution chromosome painting with repetitive and single-copy oligonucleotides in Arachis species identifies structural rearrangements and genome differentiation
| العنوان: | High-resolution chromosome painting with repetitive and single-copy oligonucleotides in Arachis species identifies structural rearrangements and genome differentiation |
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| المؤلفون: | Qin Li, Xu Jing, Lifang Zhuang, Tang Fengshou, Pei Du, Liu Hua, Caihong Cui, Xiaodong Dai, Xinyou Zhang, Zengjun Qi, Huang Bingyan, Zhang Zhongxin, Wenzhao Dong, Fu Liuyang, Li Lina, Suoyi Han, Ziqi Sun, Yonghua Han |
| المصدر: | BMC Plant Biology BMC Plant Biology, Vol 18, Iss 1, Pp 1-14 (2018) |
| سنة النشر: | 2018 |
| مصطلحات موضوعية: | 0301 basic medicine, Genome evolution, Chromosome engineering, Arachis, Arachis species, Oligonucleotides, Plant Science, Chromosomal rearrangement, Biology, Genome, DNA, Ribosomal, Chromosomes, Plant, Chromosome Painting, 03 medical and health sciences, High-resolution karyotype, lcsh:Botany, Chromosome regions, medicine, In Situ Hybridization, Fluorescence, Repetitive Sequences, Nucleic Acid, Genetics, Gene Rearrangement, medicine.diagnostic_test, Chromosome, food and beverages, Karyotype, lcsh:QK1-989, 030104 developmental biology, Karyotyping, Molecular Probes, Genomic evolution, Genome, Plant, Oligonucleotide multiplex, Fluorescence in situ hybridization, Research Article |
| الوصف: | Background Arachis contains 80 species that carry many beneficial genes that can be utilized in the genetic improvement of peanut (Arachis hypogaea L. 2n = 4x = 40, genome AABB). Chromosome engineering is a powerful technique by which these genes can be transferred and utilized in cultivated peanut. However, their small chromosomes and insufficient cytological markers have made chromosome identification and studies relating to genome evolution quite difficult. The development of efficient cytological markers or probes is very necessary for both chromosome engineering and genome discrimination in cultivated peanut. Results A simple and efficient oligonucleotide multiplex probe to distinguish genomes, chromosomes, and chromosomal aberrations of peanut was developed based on eight single-stranded oligonucleotides (SSONs) derived from repetitive sequences. High-resolution karyotypes of 16 Arachis species, two interspecific F1 hybrids, and one radiation-induced M1 plant were then developed by fluorescence in situ hybridization (FISH) using oligonucleotide multiplex, 45S and 5S rDNAs, and genomic in situ hybridization (GISH) using total genomic DNA of A. duranensis (2n = 2x = 20, AA) and A. ipaënsis (2n = 2x = 20, BB) as probes. Genomes, chromosomes, and aberrations were clearly identifiable in the established karyotypes. All eight cultivars had similar karyotypes, whereas the eight wild species exhibited various chromosomal variations. In addition, a chromosome-specific SSON library was developed based on the single-copy sequence of chromosome 6A of A. duranensis. In combination with repetitive SSONs and rDNA FISH, the single-copy SSON library was applied to identify the corresponding A3 chromosome in the A. duranensis karyotype. Conclusions The development of repetitive and single-copy SSON probes for FISH and GISH provides useful tools for the differentiation of chromosomes and identification of structural chromosomal rearrangement. It facilitates the development of high-resolution karyotypes and detection of chromosomal variations in Arachis species. To our knowledge, the methodology presented in this study demonstrates for the first time the correlation between a sequenced chromosome region and a cytologically identified chromosome in peanut. Electronic supplementary material The online version of this article (10.1186/s12870-018-1468-1) contains supplementary material, which is available to authorized users. |
| تدمد: | 1471-2229 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::11f0c57a70a7e4fa92c32f6bd1ec6880 https://pubmed.ncbi.nlm.nih.gov/30333010 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....11f0c57a70a7e4fa92c32f6bd1ec6880 |
| قاعدة البيانات: | OpenAIRE |
Find this article in full text from Springer Verlag. | تدمد: | 14712229 |
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