Cloning of an endoglycanase gene from Paenibacillus cookii and characterization of the recombinant enzyme
| العنوان: | Cloning of an endoglycanase gene from Paenibacillus cookii and characterization of the recombinant enzyme |
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| المؤلفون: | Shin Kanamasa, Suguru Shinoda, Motoo Arai |
| المصدر: | Biotechnology Letters. 34:281-286 |
| بيانات النشر: | Springer Science and Business Media LLC, 2011. |
| سنة النشر: | 2011 |
| مصطلحات موضوعية: | DNA, Bacterial, Molecular Sequence Data, Bioengineering, macromolecular substances, Cellulase, Protein Sorting Signals, Molecular cloning, Applied Microbiology and Biotechnology, Substrate Specificity, chemistry.chemical_compound, Chitin, Enzyme Stability, Chitosanase, Cloning, Molecular, Cellulose, Glucans, chemistry.chemical_classification, Chitosan, Chitosanase activity, Sequence Homology, Amino Acid, biology, Temperature, Substrate (chemistry), Sequence Analysis, DNA, General Medicine, Hydrogen-Ion Concentration, Molecular biology, Recombinant Proteins, Amino acid, Molecular Weight, carbohydrates (lipids), chemistry, Biochemistry, biology.protein, Bacillus circulans, Paenibacillus, Biotechnology |
| الوصف: | An endoglycanase gene of Paenibacillus cookii SS-24 was cloned and sequenced. This Pgl8A gene had an open reading frame of 1,230 bp that encoded a putative signal sequence (31 amino acids) and mature enzyme (378 amino acids: 41,835 Da). The enzyme was most homologous to a β-1,3-1,4-glucanase of Bacillus circulans WL-12 with 84% identity. The recombinant enzyme hydrolyzed carboxymethyl cellulose, swollen celluloses, chitosan and lichenan but not Avicel, chitin powder or xylan. With chitosan as the substrate, the optimum temperature and hydrolysis products of the recombinant enzyme varied at pH 4.0 and 8.0. This is the first report that characterizes chitosanase activity under different pH conditions. |
| تدمد: | 1573-6776 0141-5492 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::131a6185deda868bbe8e2b8c2d585f80 https://doi.org/10.1007/s10529-011-0759-5 |
| حقوق: | CLOSED |
| رقم الأكسشن: | edsair.doi.dedup.....131a6185deda868bbe8e2b8c2d585f80 |
| قاعدة البيانات: | OpenAIRE |
Find this article in full text from Springer Verlag. | تدمد: | 15736776 01415492 |
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