GATA4 regulates osteoblastic differentiation and bone remodeling via p38-mediated signaling
| العنوان: | GATA4 regulates osteoblastic differentiation and bone remodeling via p38-mediated signaling |
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| المؤلفون: | Junqing Ma, Tingting Zhou, Yuxin Zhang, Yajuan Weng, Shuyu Guo, Lin Wang |
| المصدر: | Journal of Molecular Histology. 48:187-197 |
| بيانات النشر: | Springer Science and Business Media LLC, 2017. |
| سنة النشر: | 2017 |
| مصطلحات موضوعية: | Male, 0301 basic medicine, medicine.medical_specialty, Histology, MAP Kinase Signaling System, Physiology, Bone remodeling, 03 medical and health sciences, 0302 clinical medicine, Internal medicine, medicine, Animals, Osteopontin, RNA, Small Interfering, Transcription factor, Cells, Cultured, Zinc finger transcription factor, Gene knockdown, Osteoblasts, biology, GATA4, Cell Differentiation, Osteoblast, Cell Biology, General Medicine, Immunohistochemistry, GATA4 Transcription Factor, Cell biology, Mice, Inbred C57BL, 030104 developmental biology, Endocrinology, medicine.anatomical_structure, 030220 oncology & carcinogenesis, biology.protein, Osteocalcin, Bone Remodeling |
| الوصف: | Osteoblasts play a major role in bone remodeling and are regulated by transcription factors. GATA4, a zinc finger transcription factor from the GATA family, has an unclear role in osteoblast differentiation. In this study, the role of GATA4 in osteoblast differentiation was studied both in vitro and in vivo by GATA4 knockdown. GATA4 expression increased during osteoblast differentiation. GATA4 knockdown in osteoblast precursor cells reduced alkaline phosphatase activity and decreased the formation of calcified nodule in an osteogenic-induced cell culture system. In vivo, micro-CT showed that local injection of lentivirus-delivered GATA4 shRNA caused reduced new bone formation during tooth movement. Histological analyses such as total collagen and Goldner’s trichrome staining confirmed these results. In vivo immunohistochemical analysis showed reduced expression of osterix (OSX), osteopontin (OPN), and osteocalcin (OCN) in the shGATA4 group (P |
| تدمد: | 1567-2387 1567-2379 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::131aaa214ff70717cd15a9c7924006d8 https://doi.org/10.1007/s10735-017-9719-2 |
| حقوق: | CLOSED |
| رقم الأكسشن: | edsair.doi.dedup.....131aaa214ff70717cd15a9c7924006d8 |
| قاعدة البيانات: | OpenAIRE |
Find this article in full text from Springer Verlag. | تدمد: | 15672387 15672379 |
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