NAuRA: Genomic Tool to Identify Staphylococcal Enterotoxins in Staphylococcus aureus Strains Responsible for FoodBorne Outbreaks

التفاصيل البيبلوغرافية
العنوان: NAuRA: Genomic Tool to Identify Staphylococcal Enterotoxins in Staphylococcus aureus Strains Responsible for FoodBorne Outbreaks
المؤلفون: Arnaud Felten, Yacine Titouche, Sarah Denayer, Christos Kourtis, Michel-Yves Mistou, Jacques-Antoine Hennekinne, Noémie Vingadassalon, Déborah Merda, Hristo Daskalov, Bernadette Hickey, Lucia Decastelli
المصدر: Frontiers in Microbiology
Frontiers in Microbiology, Vol 11 (2020)
بيانات النشر: Frontiers Media SA, 2020.
سنة النشر: 2020
مصطلحات موضوعية: Microbiology (medical), Genetics, Food poisoning, Phylogenetic tree, gene detection, lcsh:QR1-502, Sequence assembly, Outbreak, Biology, medicine.disease, Microbiology, Genome, lcsh:Microbiology, genomic, variant diversity, bioinformatics tool, RefSeq, medicine, Gene, staphylococcal enterotoxins genes, Original Research, Food contaminant
الوصف: Food contamination by staphylococcal enterotoxins (SEs) is responsible for many food poisoning outbreaks (FPOs) each year, and they represent the third leading cause of FPOs in Europe. SEs constitute a protein family with 27 proteins. However, enzyme immunoassays can only detect directly in food the five classical SEs (SEA-SEE). Thus, molecular characterization methods of strains found in food are now used for FPO investigations. Here, we describe the development and implementation of a genomic analysis tool called NAuRA (Nice automatic Research of alleles) that can detect the presence of 27 SEs genes in just one analysis- and create a database of allelic data and protein variants for harmonizing analyses. This tool uses genome assembly data and the 27 protein sequences of SEs. To include the different divergence levels between SE-coding genes, parameters of coverage and identity were generated from 10,000 simulations and a dataset of 244 assembled genomes from strains responsible for outbreaks in Europe as well as the RefSeq reference database. Based on phylogenetic inference performed using maximum-likelihood on the core genomes of the strains in this collection, we demonstrated that strains responsible for FPOs are distributed throughout the phylogenetic tree. Moreover, 71 toxin profiles were obtained using the NAuRA pipeline and these profiles do not follow the evolutionary history of strains. This study presents a pioneering method to investigate strains isolated from food at the genomic level and to analyze the diversity of all 27 SE-coding genes together.
تدمد: 1664-302X
URL الوصول: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::136c2cc667605e88e4197a3ea2727bfb
https://doi.org/10.3389/fmicb.2020.01483
حقوق: OPEN
رقم الأكسشن: edsair.doi.dedup.....136c2cc667605e88e4197a3ea2727bfb
قاعدة البيانات: OpenAIRE