Mitogenic lectins from Cephalosporium curvulum (CSL) and Aspergillus oryzae (AOL) mediate host–pathogen interactions leading to mycotic keratitis
| العنوان: | Mitogenic lectins from Cephalosporium curvulum (CSL) and Aspergillus oryzae (AOL) mediate host–pathogen interactions leading to mycotic keratitis |
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| المؤلفون: | Preeti Laha, Shivakumar Belur, Suhas Ballal, Shashikala R. Inamdar, Bale M. Swamy, Sanhita Roy |
| المصدر: | Molecular and Cellular Biochemistry. 434:209-219 |
| بيانات النشر: | Springer Science and Business Media LLC, 2017. |
| سنة النشر: | 2017 |
| مصطلحات موضوعية: | 0301 basic medicine, Aspergillus oryzae, Clinical Biochemistry, Microbiology, Proinflammatory cytokine, Cornea, 03 medical and health sciences, 0302 clinical medicine, Downregulation and upregulation, Lectins, Humans, Secretion, Interleukin 8, Molecular Biology, Pathogen, Keratitis, biology, Lectin, Cell Biology, General Medicine, biology.organism_classification, Acremonium, TLR2, 030104 developmental biology, Mycoses, Host-Pathogen Interactions, 030221 ophthalmology & optometry, biology.protein, Cytokines, Inflammation Mediators |
| الوصف: | A core-fucose-specific lectin, CSL from Cephalosporium curvulum, has been reported earlier. Here we assign the role for CSL and another lectin AOL, from pathogenic fungus Aspergillus oryzae, in causing mycotic keratitis. CSL and AOL show strong binding to immortalized and primary human corneal epithelial cells (HCECs) which are inhibited by asialofetuin, confirming their glycan-mediated binding. CSL and AOL showed increase in viability at lower concentrations (0.07 µg/ml) whereas at higher concentrations (0.15 µg/ml and 0.30 µg/ml), have inhibitory effect on immortalized HCECs. Lectin-mediated effect was comparable with the effect induced by the Colony Forming Units (CFUs) of C. curvulum and A. oryzae. CFUs induced more than 1.5-fold increase in HCECs proliferation. Both lectins and fungal CFUs induce secretion of proinflammatory cytokines IL6 and IL8 implicated in ocular diseases. This was supported by upregulation of TLR2 and 4 by lectins as revealed by flow cytometry and RT-PCR. CSL and AOL mediate host-pathogen interactions leading to mycotic keratitis. The mechanism of pathogenesis is possibly initiated through surface binding of mycelia through the lectins to TLR2/4 followed by upregulation of proinflammatory cytokines IL6, IL8 and TLR2 and 4. Understanding the mechanism of pathogenesis is of clinical significance in designing and developing therapeutic strategy to control the infection. |
| تدمد: | 1573-4919 0300-8177 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::13c74268f393ea05654ec36a7103b234 https://doi.org/10.1007/s11010-017-3050-9 |
| حقوق: | CLOSED |
| رقم الأكسشن: | edsair.doi.dedup.....13c74268f393ea05654ec36a7103b234 |
| قاعدة البيانات: | OpenAIRE |
Find this article in full text from Springer Verlag. | تدمد: | 15734919 03008177 |
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