Wt1 transcription factor impairs cardiomyocyte specification and drives a phenotypic switch from myocardium to epicardium
| العنوان: | Wt1 transcription factor impairs cardiomyocyte specification and drives a phenotypic switch from myocardium to epicardium |
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| المؤلفون: | Marques, Ines J., Ernst, Alexander, Arora, Prateek, Vianin, Andrej, Hetke, Tanja, Sanz-Morej��n, Andr��s, Naumann, Uta, Odriozola, Adolfo, Langa, Xavier, Andr��s-Delgado, Laura, Zuber, Beno��t, Torroja, Carlos, Osterwalder, Marco, Sim��es, Filipa, Englert, Christoph, Mercader, Nadia |
| المساهمون: | European Research Council, Instituto de Salud Carlos III, Ministerio de Ciencia e Innovación (España), Fundación ProCNIC, Ministerio de Ciencia e Innovación. Centro de Excelencia Severo Ochoa (España), ERA-NET |
| المصدر: | Development Marques, Ines J; Ernst, Alexander; Arora, Prateek; Vianin, Andrej; Hetke, Tanja; Sanz-Morejon, Andrés; Naumann, Uta; Odriozola, Adolfo; Langa, Xavier; Andrés-Delgado, Laura; Zuber, Benoît; Torroja, Carlos; Osterwalder, Marco; Simões, Filipa; Englert, Christoph; Mercader, Nadia (2022). WT1 transcription factor impairs cardiomyocyte specification and drives a phenotypic switch from myocardium to epicardium. Development, 149(6) The Company of Biologists 10.1242/dev.200375 <http://dx.doi.org/10.1242/dev.200375> |
| بيانات النشر: | The Company of Biologists Ltd, 2022. |
| سنة النشر: | 2022 |
| مصطلحات موضوعية: | Myocardium, Gene Expression Regulation, Developmental, 610 Medicine & health, Zebrafish Proteins, Animals, Myocytes, Cardiac, wt1a, wt1b, heart development, cardiomyocyte, epicardium, zebrafish, cell fate, WT1 Proteins, Molecular Biology, Pericardium, Zebrafish, Developmental Biology, Transcription Factors, Research Article |
| الوصف: | During development, the heart grows by addition of progenitor cells to the poles of the primordial heart tube. In the zebrafish, Wilms tumor 1 transcription factor a (wt1a) and b (wt1b) genes are expressed in the pericardium, at the venous pole of the heart. From this pericardial layer, the proepicardium emerges. Proepicardial cells are subsequently transferred to the myocardial surface and form the epicardium, covering the myocardium. We found that while wt1a and wt1b expression is maintained in proepicardial cells, it is downregulated in pericardial cells that contributes cardiomyocytes to the developing heart. Sustained wt1b expression in cardiomyocytes reduced chromatin accessibility of specific genomic loci. Strikingly, a subset of wt1a- and wt1b-expressing cardiomyocytes changed their cell-adhesion properties, delaminated from the myocardium and upregulated epicardial gene expression. Thus, wt1a and wt1b act as a break for cardiomyocyte differentiation, and ectopic wt1a and wt1b expression in cardiomyocytes can lead to their transdifferentiation into epicardial-like cells. NM has been funded by SNF grant 320030E-164245 and ERC Consolidator grant 2018 819717. The CNIC is supported by the Instituto de Salud Carlos III (ISCIII), the Ministerio de Ciencia e Innovación (MCIN) and the Pro CNIC Foundation and is a Severo Ochoa Center of Excellence (SEV-2015-0505). Benoît Zuber is supported by SNF grant 179520 and ERA-NET NEURON grant 185536. M.O. was supported by SNF grant PCEFP3_186993. Sí |
| وصف الملف: | application/pdf |
| اللغة: | English |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::13e42717846f734a84ad0c98e83fc8c9 https://europepmc.org/articles/PMC8977101/ |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....13e42717846f734a84ad0c98e83fc8c9 |
| قاعدة البيانات: | OpenAIRE |
| الوصف غير متاح. |