Cellular Stemness Maintenance of Human Adipose‐Derived Stem Cells on ZnO Nanorod Arrays
| العنوان: | Cellular Stemness Maintenance of Human Adipose‐Derived Stem Cells on ZnO Nanorod Arrays |
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| المؤلفون: | Hong Liu, Yuanhua Sang, Jiazhi Duan, Shuhua Wang, Feng Liu, Duo Chen, Jian-Jun Wang, Dong Li, Ying Kong, Shan Zhang, Yuan Huang, Baojin Ma |
| المصدر: | Small. 15:1904099 |
| بيانات النشر: | Wiley, 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | Kruppel-Like Transcription Factors, Adipose tissue, 02 engineering and technology, Cell fate determination, 010402 general chemistry, 01 natural sciences, Biomaterials, Cell therapy, Kruppel-Like Factor 4, Humans, General Materials Science, Nanotopography, Cells, Cultured, Cell Proliferation, Nanotubes, Chemistry, Cell growth, Stem Cells, Cell Differentiation, General Chemistry, 021001 nanoscience & nanotechnology, In vitro, 0104 chemical sciences, Cell biology, Adipose Tissue, KLF4, Zinc Oxide, Stem cell, 0210 nano-technology, Biotechnology |
| الوصف: | Ever-growing tissue regeneration and other stem cell therapies cause pressing need for large population of self-renewable stem cells. However, stem cells gradually lose their stemness after long-term in vitro cultivation. In this study, a ZnO nanorod (ZnO NR) array is used to maintain the stemness of human adipose-derived stem cells (hADSCs). The results prove that after culturing hADSCs on ZnO NRs for 3 weeks, the stemness genes and protein expression level are higher than that on culture plates and ZnO film. ZnO NRs can maintain stemness of hADSCs without inhibiting the cell proliferation and oriented differentiation capabilities. KLF4 (Kruppel-like factor 4) is a Zn2+ -binding gene that plays a vital role in cell proliferation and differentiation. Sustained Zn2+ release and the increased expression of KLF4 can be detected, suggesting that ZnO NRs have efficiently released Zn2+ for stemness maintenance. Taken together, the nanotopography of ZnO NRs and the Zn2+ release synergistically facilitate stemness maintenance. This study has provided a powerful tool for directing cell fate, maintaining stemness, and realizing the expansion of stem cells in vitro, which will open a new route for the manufacture of large populations of stem cells and fulfilling the growing demand for the cell therapy market. |
| تدمد: | 1613-6829 1613-6810 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::143e78f77fa7a1d41b5d196018161aa0 https://doi.org/10.1002/smll.201904099 |
| حقوق: | CLOSED |
| رقم الأكسشن: | edsair.doi.dedup.....143e78f77fa7a1d41b5d196018161aa0 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 16136829 16136810 |
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