Recombinant MVA-prime elicits neutralizing antibody responses by inducing antigen-specific B cells in the germinal center
| العنوان: | Recombinant MVA-prime elicits neutralizing antibody responses by inducing antigen-specific B cells in the germinal center |
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| المؤلفون: | Laura L. Sutherland, Takuya Yamamoto, Barton F. Haynes, Norman L. Letvin, Madhu Prabhakaran, Wesley Hoyland, Sandeep Narpala, David J. Pickup, Steven Ma, Christopher Fletez-Brant, Mario Roederer, Sampa Santra, Adrian B. McDermott, Hua-Xin Liao, Richard A. Koup, Michelle L. Lifton, Elizabeth Ramsburg, Kristin L. Boswell, Gail Levine, Constantinos Petrovas, Leila Eslamizar, David J. Leggat, Kathryn Furr, David C. Montefiori |
| المصدر: | NPJ Vaccines npj Vaccines, Vol 6, Iss 1, Pp 1-10 (2021) |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | 0301 basic medicine, Live attenuated vaccines, Immunology, Priming (immunology), Article, law.invention, 03 medical and health sciences, chemistry.chemical_compound, 0302 clinical medicine, law, Pharmacology (medical), Neutralizing antibody, RC254-282, Pharmacology, Vaccines, biology, Vaccine trial, Germinal center, Neoplasms. Tumors. Oncology. Including cancer and carcinogens, RC581-607, Virology, Titer, 030104 developmental biology, chemistry, biology.protein, Recombinant DNA, Infectious diseases, Lymph, Immunologic diseases. Allergy, DNA, 030215 immunology |
| الوصف: | The RV144 HIV-1 vaccine trial has been the only clinical trial to date that has shown any degree of efficacy and associated with the presence of vaccine-elicited HIV-1 envelope-specific binding antibody and CD4+ T-cell responses. This trial also showed that a vector-prime protein boost combined vaccine strategy was better than when used alone. Here we have studied three different priming vectors—plasmid DNA, recombinant MVA, and recombinant VSV, all encoding clade C transmitted/founder Env 1086 C gp140, for priming three groups of six non-human primates each, followed by a protein boost with adjuvanted 1086 C gp120 protein. Our data showed that MVA-priming favors the development of higher antibody binding titers and neutralizing activity compared with other vectors. Analyses of the draining lymph nodes revealed that MVA-prime induced increased germinal center reactivity characterized by higher frequencies of germinal center (PNAhi) B cells, higher frequencies of antigen-specific B-cell responses as well as an increased frequency of the highly differentiated (ICOShiCD150lo) Tfh-cell subset. |
| تدمد: | 2059-0105 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::14b4872fd248bac882951b8abd1964cd https://pubmed.ncbi.nlm.nih.gov/33495459 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....14b4872fd248bac882951b8abd1964cd |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 20590105 |
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