It’s All About Timing: Early Feeding Promotes Intestinal Maturation by Shifting the Ratios of Specialized Epithelial Cells in Chicks
| العنوان: | It’s All About Timing: Early Feeding Promotes Intestinal Maturation by Shifting the Ratios of Specialized Epithelial Cells in Chicks |
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| المؤلفون: | Tal Melkman-Zehavi, Zehava Uni, Jonathan Dayan, Naama Reicher |
| المصدر: | Frontiers in Physiology, Vol 11 (2020) Frontiers in Physiology |
| بيانات النشر: | Frontiers Media S.A., 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | 0301 basic medicine, animal structures, Enterocyte, Physiology, Cellular differentiation, Crypt, In situ hybridization, Biology, first feeding, lcsh:Physiology, Andrology, 03 medical and health sciences, Physiology (medical), medicine, intestine, Original Research, lcsh:QP1-981, maturation, 0402 animal and dairy science, 04 agricultural and veterinary sciences, 040201 dairy & animal science, Epithelium, Small intestine, epithelial cells, chick, 030104 developmental biology, medicine.anatomical_structure, embryonic structures, Specialized Epithelial Cell, Stem cell |
| الوصف: | The small intestine (SI) of chicks (Gallus gallus) matures rapidly during the initial post-hatch period and acquires digestive, absorptive, and secretive capabilities. The effects of the timing of first feeding on the quantities and distribution of specialized epithelial cells, which generate and maintain SI morphology and functionality, have not yet been examined. In this study, we identified specialized SI epithelial cell sub-types, including stem, progenitor, proliferating, and differentiated cells within crypts and villi of chicks during the first 10 days post-hatch, byin situhybridization (ISH), immunofluorescence (IF), and histochemical staining. We then examined their quantities and ratios between day of hatch and d10 in chicks that were fed upon hatch [early feeding (EF)], compared to chicks that were fed 24 h post-hatch [delayed feeding (DF)]. Results showed that EF increased total cell quantities in the crypts and villi at days 1, 3, 7, and 10, compared to DF (p< 0.0001). At d3, EF, in comparison to DF, decreased crypt stem cell proportions (p< 0.0001), increased crypt proliferating (p< 0.01) and differentiated (p< 0.05) cell proportions, and increased villus enterocyte proportions (p< 0.01). By d10, EF increased both the quantities and proportions of villus enterocytes and goblet cells, compared to DF. We conclude that feeding upon hatch, compared to 24 h-delayed feeding, enhanced SI maturation and functionality by increasing the quantities and proportions of proliferating and differentiated cells, thus expanding the digestive, absorptive, and secretive cell populations throughout the initial post-hatch period. |
| اللغة: | English |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1517358f1f32b44a7bce2aab6f90a44c https://www.frontiersin.org/articles/10.3389/fphys.2020.596457/full |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....1517358f1f32b44a7bce2aab6f90a44c |
| قاعدة البيانات: | OpenAIRE |
| الوصف غير متاح. |