miR-458b-5p regulates ovarian granulosa cells proliferation through Wnt/β‐catenin signaling pathway by targeting catenin beta-1
| العنوان: | miR-458b-5p regulates ovarian granulosa cells proliferation through Wnt/β‐catenin signaling pathway by targeting catenin beta-1 |
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| المؤلفون: | Shen Zhang, Xu Han, Wenwen Wang, Jun Teng, Qin Zhang, Hui Tang |
| المصدر: | Animal Bioscience Animal Bioscience, Vol 34, Iss 6, Pp 957-966 (2021) |
| بيانات النشر: | Asian Australasian Association of Animal Production Societies, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | endocrine system, Catenin Beta-1 (CTNNB1), Granulosa Cells, Proliferation, Wnt, Physiology, Article, miR-458b-5p, chemistry.chemical_compound, Genetics, Propidium iodide, catenin beta-1 (), granulosa cells, proliferation, wnt, β-catenin Signaling Pathway, General Veterinary, biology, Chemistry, CD44, 0402 animal and dairy science, Wnt signaling pathway, 04 agricultural and veterinary sciences, Transfection, Cell cycle, Animal Breeding and Genetics, 040201 dairy & animal science, Cell biology, QL1-991, Catenin, Catenin Beta-1, biology.protein, Animal Science and Zoology, Signal transduction, Zoology, Food Science |
| الوصف: | Objective: Ovarian follicular development, which dependent on the proliferation and differentiation of granulosa cells (GCs), is a complex biological process in which miRNA plays an important role. Our previous study showed that miR-458b-5p is associated with ovarian follicular development in chicken. The detailed function and molecular mechanism of miR-458b-5p in GCs is unclear.Methods: The luciferase reporter assay was used to verify the targeting relationship between miR-458b-5p and catenin beta-1 (CTNNB1), which is an important transcriptional regulatory factor of the Wnt/β-catenin pathway. The cell counting kit-8 (CCK-8) assay, flow cytometry with propidium iodide (PI) and annexin V-fluorescein isothiocyanate (FITC) labeling were applied to explore the effect of miR-458b-5p on proliferation, cell cycle and apoptosis of chicken GCs. Quantitative real-time polymerase chain reaction and Western blot were used to detect the mRNA and protein expression levels.Results: We demonstrated that the expression of miR-458b-5p and CTNNB1 showed the opposite relationship in GCs and theca cells of hierarchical follicles. The luciferase reporter assay confirmed that CTNNB1 is the direct target of miR-458b-5p. Using CCK-8 assay and flow cytometry with PI and Annexin V-FITC labeling, we observed that transfection with the miR-458b-5p mimics significantly reduced proliferation and has no effects on apoptosis of chicken GCs. In addition, miR-458b-5p decreased the mRNA and protein expression of CD44 molecule and matrix metallopeptidase 7, which are the downstream effectors of CTNNB1 in Wnt/β-Catenin pathway and play functional roles in cell proliferation.Conclusion: Taken together, the data indicate that miR-458b-5p regulates ovarian GCs proliferation through Wnt/β-catenin signaling pathway by targeting CTNNB1, suggesting that miR-458b-5p and its target gene CTNNB1 may potentially play a role in chicken ovarian follicular development. |
| تدمد: | 2765-0235 2765-0189 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::15335e80aebd8e7aa75eb4af74bad419 https://doi.org/10.5713/ajas.20.0392 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....15335e80aebd8e7aa75eb4af74bad419 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 27650235 27650189 |
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