Mitogen-responsive expression of RhoB is regulated by RNA stability
| العنوان: | Mitogen-responsive expression of RhoB is regulated by RNA stability |
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| المؤلفون: | Ivan Sadowski, Tom Malcolm, Elham Ettehadieh |
| المصدر: | Oncogene. 22:6142-6150 |
| بيانات النشر: | Springer Science and Business Media LLC, 2003. |
| سنة النشر: | 2003 |
| مصطلحات موضوعية: | Untranslated region, Cancer Research, RNA Stability, RHOB, Molecular Sequence Data, Biology, Transfection, Mice, Gene expression, Genetics, Animals, Amino Acid Sequence, Northern blot, Promoter Regions, Genetic, rhoB GTP-Binding Protein, Molecular Biology, AU-rich element, Reporter gene, Messenger RNA, Base Sequence, Epidermal Growth Factor, Sequence Homology, Amino Acid, 3T3 Cells, Fibroblasts, Molecular biology, Rats, Mitogens, Immediate early gene, DNA Damage |
| الوصف: | The small GTPase-encoding gene RhoB is strongly induced as part of the immediate early response of serum-stimulated fibroblasts. In this report, we have characterized the mechanism for growth factor responsiveness of RhoB in Rat-2 fibroblasts. By Northern blotting and ribonuclease protection, we observed low or barely detectable levels of RhoB mRNA in quiescent cells, but expression was transiently induced in response to serum stimulation, such that the mRNA peaked within 30 min and then declined over the next hour. Analysis of the rat promoter revealed cis-elements conserved with the mouse and human genes, including a pair of CEBP sites near the transcriptional start site. However, in contrast to the analysis of RNA, RhoB promoter fusions were constitutively expressed in quiescent cells in transient transfections, and were unaffected by serum. Similarly, stable RhoB promoter integrants were highly expressed in quiescent cells, and growth factor caused a slight decrease in activity. This indicates that growth factor-inducible RhoB expression cannot be mediated by transcriptional activation. We then examined decay of the RhoB mRNA and found that serum caused significant stabilization. Additionally, fusion of the 3' RhoB untranslated region (UTR) to a constitutively expressed reporter gene caused serum and growth factor as well as DNA damage-inducible expression. These observations are consistent with the view that RhoB mRNA is produced constitutively but its abundance is controlled in response to growth factors, and other signals including DNA damage, by stabilization through elements within the 3' UTR. |
| تدمد: | 1476-5594 0950-9232 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::154700c5ec06021d573f8586bc7f0fc2 https://doi.org/10.1038/sj.onc.1206638 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....154700c5ec06021d573f8586bc7f0fc2 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14765594 09509232 |
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