Sciatic nerve cuffing in mice: A model of sustained neuropathic pain
| العنوان: | Sciatic nerve cuffing in mice: A model of sustained neuropathic pain |
|---|---|
| المؤلفون: | Elisabeth Waltisperger, Michel Barrot, V. Pallage, Malika Benbouzid, Mathieu Rajalu, Pierrick Poisbeau, Marie José Freund-Mercier, Stéphane Doridot |
| المساهمون: | Institut des Neurosciences Cellulaires et Intégratives (INCI), Université Louis Pasteur - Strasbourg I-Centre National de la Recherche Scientifique (CNRS) |
| المصدر: | European Journal of Pain European Journal of Pain, Wiley, 2008, 12 (5), pp.591--599. ⟨10.1016/j.ejpain.2007.10.002⟩ |
| بيانات النشر: | Wiley, 2008. |
| سنة النشر: | 2008 |
| مصطلحات موضوعية: | Male, Narcotics, Genetically modified mouse, Emotions, Video Recording, Motor Activity, Mice, Escape Reaction, Animals, Medicine, Maze Learning, Morphine, business.industry, Anti-Inflammatory Agents, Non-Steroidal, Social Behavior Disorders, Drug Tolerance, medicine.disease, Anxiety Disorders, Constriction, Mice, Inbred C57BL, Anesthesiology and Pain Medicine, Nociception, Peripheral neuropathy, Allodynia, Hyperalgesia, Ketoprofen, Anesthesia, Models, Animal, Neuropathic pain, Cuff, [SDV.NEU]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC], Stress, Mechanical, Sciatic nerve, Sciatic Neuropathy, medicine.symptom, business, medicine.drug |
| الوصف: | International audience; Because of its severity, chronicity, resistance to usual therapy and its consequences on quality of life, neuropathic pain represents a real clinical challenge. Fundamental research on this pathology uses metabolic, pharmacological or traumatic models in rodents that reproduce the characteristic human pain symptoms. In 1996, Mosconi and Kruger morphologically described a model of peripheral neuropathy in which a cuff of polyethylene tubing was placed around the sciatic nerve in rats. In the present study, we evaluated the behavioral consequences of this neuropathic pain model in C57Bl/6J mice which is the main genetic background used for studies in transgenic mice. A short cuff of polyethylene tubing was unilaterally placed around the main branch of the sciatic nerve. It induced an ipsilateral heat thermal hyperalgesia lasting around 3 weeks, and a sustained ipsilateral mechanical allodynia lasting at least 2 months. We showed that this neuropathic pain model is insensitive to ketoprofen, a non-steroidal anti-inflammatory drug. Morphine treatment acutely suppressed the mechanical allodynia, but tolerance to this effect rapidly developed. The analysis of video recordings revealed that most aspects of spontaneous behavior remained unaffected on the long term, excepted for a decrease in the time spent at social interaction for the neuropathic mice. Using the elevated plus-maze and the marble-burying test, we also showed that neuropathic mice develop an anxiety phenotype. Our data indicate that sciatic nerve cuffing in mice is a pertinent model for the study of nociceptive and emotional consequences of sustained neuropathic pain. |
| تدمد: | 1090-3801 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::155bfe301d209b45b415bb697a3a889e https://doi.org/10.1016/j.ejpain.2007.10.002 |
| حقوق: | CLOSED |
| رقم الأكسشن: | edsair.doi.dedup.....155bfe301d209b45b415bb697a3a889e |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 10903801 |
|---|