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Construction of an integrated pepper map using RFLP, SSR, CAPS, AFLP, WRKY, rRAMP, and BAC end sequences

التفاصيل البيبلوغرافية
العنوان: Construction of an integrated pepper map using RFLP, SSR, CAPS, AFLP, WRKY, rRAMP, and BAC end sequences
المؤلفون: Heung-Ryul Lee, Soung-Woo Park, Jung-Heon Han, Byung-Dong Kim, Ik-Hyun Bae, Ki-Taek Kim, Woong-Ki Min, Hyoun-Joung Kim
المصدر: Molecules and Cells. 27:21-37
بيانات النشر: Springer Science and Business Media LLC, 2008.
سنة النشر: 2008
مصطلحات موضوعية: Genetic Markers, Genetics, Chromosomes, Artificial, Bacterial, Physical Chromosome Mapping, food and beverages, Minisatellite Repeats, Sequence Analysis, DNA, Cell Biology, General Medicine, Biology, WRKY protein domain, Species Specificity, Gene mapping, Genetic marker, Pepper, Microsatellite, Amplified fragment length polymorphism, Amplified Fragment Length Polymorphism Analysis, Restriction fragment length polymorphism, Capsicum, Molecular Biology, Polymorphism, Restriction Fragment Length, Microsatellite Repeats
الوصف: Map-based cloning to find genes of interest, markerassisted selection (MAS), and marker-assisted breeding (MAB) all require good genetic maps with high reproducible markers. For map construction as well as chromosome assignment, development of single copy PCR-based markers and map integration process are necessary. In this study, the 132 markers (57 STS from BAC-end sequences, 13 STS from RFLP, and 62 SSR) were newly developed as single copy type PCR-based markers. They were used together with 1830 markers previously developed in our lab to construct an integrated map with the Joinmap 3.0 program. This integrated map contained 169 SSR, 354 RFLP, 23 STS from BAC-end sequences, 6 STS from RFLP, 152 AFLP, 51 WRKY, and 99 rRAMP markers on 12 chromosomes. The integrated map contained four genetic maps of two interspecific (Capsicum annuum 'TF68' and C. chinense 'Habanero') and two intraspecific (C. annuum 'CM334' and C. annuum 'Chilsungcho') populations of peppers. This constructed integrated map consisted of 805 markers (map distance of 1858 cM) in interspecific populations and 745 markers (map distance of 1892 cM) in intraspecific populations. The used pepper STS were first developed from end sequences of BAC clones from Capsicum annuum 'CM334'. This integrated map will provide useful information for construction of future pepper genetic maps and for assignment of linkage groups to pepper chromosomes.
تدمد: 0219-1032
1016-8478
URL الوصول: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::15728f5957ce7221fb514ac3b600fd5e
https://doi.org/10.1007/s10059-009-0002-6
حقوق: CLOSED
رقم الأكسشن: edsair.doi.dedup.....15728f5957ce7221fb514ac3b600fd5e
قاعدة البيانات: OpenAIRE
الوصف
تدمد:02191032
10168478