First molecular detection of Toxoplasma gondii in vegetable samples in China using qualitative, quantitative real-time PCR and multilocus genotyping
| العنوان: | First molecular detection of Toxoplasma gondii in vegetable samples in China using qualitative, quantitative real-time PCR and multilocus genotyping |
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| المؤلفون: | Xiuping Li, Panagiotis Karanis, Liqing Ma, Anna Lass, Ioannis Kontogeorgos, Xueyong Zhang |
| المصدر: | Scientific Reports, Vol 9, Iss 1, Pp 1-11 (2019) Scientific Reports |
| بيانات النشر: | Nature Publishing Group, 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | 0301 basic medicine, Veterinary medicine, China, 030231 tropical medicine, Pcr assay, lcsh:Medicine, Real-Time Polymerase Chain Reaction, Article, 03 medical and health sciences, 0302 clinical medicine, Food Parasitology, Vegetables, parasitic diseases, lcsh:Science, Multidisciplinary, biology, lcsh:R, Toxoplasma gondii, Contamination, DNA, Protozoan, biology.organism_classification, Multilocus genotyping, 030104 developmental biology, Quantitative Real Time PCR, Risk factors, Parasitology, lcsh:Q, Toxoplasma, Multilocus Sequence Typing |
| الوصف: | Toxoplasma gondii infection is becoming increasing problem in China but there is no data concerning contamination of vegetables intended for consumption with this parasite. The aim of the present study was to investigate fresh vegetables originated from open markets located in the Xining City, the Qinghai-Tibet Plateau (QTP), P.R. China for their contamination with T. gondii. A total of 279 fresh vegetable samples were collected and analysed using real-time PCR assay targeting B1 gene and multilocus genotyping. T. gondii DNA was found in 10 (3.6%) samples tested; eight of them represented T. gondii type I and remaining two T. gondii type II. The approximate level of contamination of positive vegetables samples, estimated based on quantitative real-time PCR (qPCR), ranged between less than one and 27000 T. gondii oocysts per sample, with majority not exceeding several oocysts per sample. The results of the study confirmed that T. gondii is present in vegetables offered in open markets in the Qinghai province, P.R. China; eating them unwashed and raw may therefore pose a threat to consumers. This is the first investigation describing T. gondii detection in fresh vegetables intended for consumption collected from the territory of P.R. China using sensitive molecular tools. |
| اللغة: | English |
| تدمد: | 2045-2322 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1612262f706049caa326dc428cc5bb0e http://link.springer.com/article/10.1038/s41598-019-54073-6 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....1612262f706049caa326dc428cc5bb0e |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 20452322 |
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