Two new Salmonella genomic islands 1 from Proteus mirabilis and description of blaCTX-M-15 on a variant (SGI1-K7)
| العنوان: | Two new Salmonella genomic islands 1 from Proteus mirabilis and description of blaCTX-M-15 on a variant (SGI1-K7) |
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| المؤلفون: | Catherine Neuwirth, Claire de Curraize, Eliane Siebor, Julien Bador, Lucie Amoureux, Angélique Chapuis |
| المصدر: | Journal of Antimicrobial Chemotherapy. 73:1804-1807 |
| بيانات النشر: | Oxford University Press (OUP), 2018. |
| سنة النشر: | 2018 |
| مصطلحات موضوعية: | DNA, Bacterial, 0301 basic medicine, Microbiology (medical), Transposable element, Salmonella, Genomic Islands, 030106 microbiology, Biology, Integron, medicine.disease_cause, Polymerase Chain Reaction, beta-Lactamases, Integrons, Transposition (music), 03 medical and health sciences, symbols.namesake, Plasmid, Drug Resistance, Multiple, Bacterial, medicine, Humans, Pharmacology (medical), Proteus mirabilis, Pharmacology, Sanger sequencing, Genetics, Whole Genome Sequencing, Genetic Variation, biology.organism_classification, Enterobacteriaceae, Anti-Bacterial Agents, Hospitalization, Infectious Diseases, Genes, Bacterial, symbols, biology.protein, France, Proteus Infections, Plasmids |
| الوصف: | Objectives To characterize the structure of Salmonella genomic islands 1 (SGI1s) from two clinical Proteus mirabilis isolates: one producing an ESBL and the other a penicillinase. Methods WGS completed by PCR and Sanger sequencing was performed to determine sequences of SGI1s from Pm2CHAMA and Pm37THOMI strains. Results Two new variants of SGI1 named SGI1-Pm2CHAMA (53.6 kb) and SGI1-K7 (55.1 kb) were identified. The backbone of SGI1-Pm2CHAMA shared 99.9% identity with that of SGI1. Its MDR region (26.3 kb) harboured two class 1 integrons (an In2-type integron and an In4-type integron) containing in particular a qacH cassette (encoding a quaternary ammonium compound efflux pump). These two integrons framed a complex region (harbouring among others blaCARB-4) resulting from transposon insertions mediated by IS26 and successive transposition events of ISs (ISAba14 isoform and the new ISPmi2). The second variant (SGI1-K7) had the same backbone as SGI1-K. Its MDR region (29.7 kb) was derived from that of SGI1-K and was generated by three events. The two main events were mediated by IS26: inversion of a large portion of the MDR region of SGI1-K and insertion of a structure previously reported on plasmids carried by prevalent and successful MDR clones of Enterobacteriaceae. This last event led to the insertion of the blaCTX-M-15 gene into SGI1-K7. Conclusions This study confirmed the great plasticity of the MDR region of SGI1 and its potential key role for the dissemination of clinically significant antibiotic resistance among Enterobacteriaceae. |
| تدمد: | 1460-2091 0305-7453 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::163c8af4fd1e111ba99183ab7e362d7b https://doi.org/10.1093/jac/dky108 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....163c8af4fd1e111ba99183ab7e362d7b |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14602091 03057453 |
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