The multivesicular body is the major internal site of prion conversion
| العنوان: | The multivesicular body is the major internal site of prion conversion |
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| المؤلفون: | Yang-In Yim, Rajgopal Yadavalli, Lois E. Greene, Xiaohong Zhao, Evan Eisenberg, Bum-Chan Park |
| المصدر: | Journal of Cell Science |
| بيانات النشر: | The Company of Biologists, 2015. |
| سنة النشر: | 2015 |
| مصطلحات موضوعية: | PrPSc Proteins, Amyloid, Prions, animal diseases, Endocytic cycle, macromolecular substances, Biology, Mice, Lysosome, medicine, Animals, TSG101, Multivesicular body, PrPC Proteins, Multivesicular Body, Multivesicular Bodies, Brain, Conversion, Cell Biology, nervous system diseases, Cell biology, Retromer complex, medicine.anatomical_structure, nervous system, RAB7A, Prion, Lysosomes, Protein Processing, Post-Translational, Research Article, Scrapie |
| الوصف: | The conversion of the properly folded prion protein, PrPc, to its misfolded amyloid form, PrPsc, occurs as the two proteins traffic along the endocytic pathway and PrPc is exposed to PrPsc. To determine the specific site of prion conversion, we knocked down various proteins in the endocytic pathway including Rab7a, Tsg101 and Hrs (also known as HGS). PrPsc was markedly reduced in two chronically infected cell lines by preventing the maturation of the multivesicular body, a process that begins in the early endosome and ends with the sorting of cargo to the lysosome. By contrast, knocking down proteins in the retromer complex, which diverts cargo away from the multivesicular body caused an increase in PrPsc levels. These results suggest that the multivesicular body is the major site for intracellular conversion of PrPc to PrPsc. |
| تدمد: | 1477-9137 0021-9533 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1660c676b4896293d5f3f0e41291b622 https://doi.org/10.1242/jcs.165472 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....1660c676b4896293d5f3f0e41291b622 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14779137 00219533 |
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