Phenotypic diversity and selection maintain Leishmania amazonensis infectivity in BALB/c mouse model
| العنوان: | Phenotypic diversity and selection maintain Leishmania amazonensis infectivity in BALB/c mouse model |
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| المؤلفون: | Gilles Merlin, Benoît Espiau, Armelle Cuvillier, Aldina Barral, Virginia Vilhena |
| المساهمون: | Laboratoire de Génomique Fonctionnelle des Trypanosomatidés, Université de Bordeaux (UB), Laboratoire d'Excellence CORAIL (LabEX CORAIL), Institut de Recherche pour le Développement (IRD)-Université des Antilles et de la Guyane (UAG)-École des hautes études en sciences sociales (EHESS)-École pratique des hautes études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de La Réunion (UR)-Université de la Polynésie Française (UPF)-Université de la Nouvelle-Calédonie (UNC)-Institut d'écologie et environnement-Université des Antilles (UA), Fundação Oswaldo Cruz (FIOCRUZ), Réseau International des Instituts Pasteur (RIIP), Instituto de Investigação em Imunologia |
| المصدر: | Memórias do Instituto Oswaldo Cruz, Volume: 112, Issue: 1, Pages: 44-52, Published: JAN 2017 Memórias do Instituto Oswaldo Cruz Memórias do Instituto Oswaldo Cruz, Instituto Oswaldo Cruz, Ministério da Saúde, 2017, Phenotypic diversity and selection maintain Leishmania amazonensis infectivity in BALB/c mouse model, 112 (1), ⟨10.1590/0074-02760160280⟩ Memórias do Instituto Oswaldo Cruz., Vol 112, Iss 1, Pp 44-52 |
| بيانات النشر: | Instituto Oswaldo Cruz, Ministério da Saúde, 2017. |
| سنة النشر: | 2017 |
| مصطلحات موضوعية: | 0301 basic medicine, Microbiology (medical), Time Factors, lcsh:Arctic medicine. Tropical medicine, BALB/c Mouse, lcsh:RC955-962, Leishmania mexicana, 030231 tropical medicine, lcsh:QR1-502, Clone (cell biology), Leishmaniasis, Cutaneous, selection, Biology, lcsh:Microbiology, Lesion, Mice, 03 medical and health sciences, 0302 clinical medicine, In vivo, medicine, Animals, [SDV.MP.PAR]Life Sciences [q-bio]/Microbiology and Parasitology/Parasitology, Infectivity, Mice, Inbred BALB C, infectivity, Articles, Leishmania, biology.organism_classification, Phenotype, Virology, Disease Models, Animal, 030104 developmental biology, [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology, subcloning, Cosmid, Female, medicine.symptom, phenotypic diversity, Leishmania amazonensis |
| الوصف: | International audience; Leishmania are protozoan parasites that show remarkable diversity, as revealed by the various clinical forms of leishmaniasis,which can range from mild skin lesions to severe metastatic cutaneous/mucosal lesions. The exact nature andextent of Leishmania phenotypic diversity in establishing infection is not fully understood. In order to try to understandsome aspects of this diversity, we subcutaneously infected BALB/c mice with first and second generation subclones of aL. amazonensis strain isolated from a patient (BA125) and examined in vivo lesion growth rate and antimony susceptibility.In vivo fast-, medium- and slow-growing subclones were obtained; moreover, fast-growing subclones could generateslow-growing subclones and inversely, revealing the continuous generation of diversity after passage into mice. Noantimony-resistant subclone appeared, probably a rare occurrence. By tagging subclone cells with a L. amazonensisgenomic cosmid library, we found that only a very small number of founding cells could produce lesions. Leishmaniaclones transfected with in vivo selected individual cosmids were also diverse in terms of lesion growth rate, revealing thecosmid-independent intrinsic characteristics of each clone. Our results suggest that only a few of the infecting parasitesare able to grow and produce lesions; later, within the cell mixture of each lesion, there coexist several parasite populationswith different potentialities to grow lesions during the next infection round. This may reflect a sort of programmedheterogeneity of individual parasites, favoring the survival of some individuals in various environmental conditions. |
| وصف الملف: | text/html |
| اللغة: | English |
| تدمد: | 0074-0276 1678-8060 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::16bb396d235740bbdf42c48c19766944 http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762017000100044&lng=en&tlng=en |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....16bb396d235740bbdf42c48c19766944 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 00740276 16788060 |
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