JC Virus Agnoprotein Inhibits In Vitro Differentiation of Oligodendrocytes and Promotes Apoptosis
| العنوان: | JC Virus Agnoprotein Inhibits In Vitro Differentiation of Oligodendrocytes and Promotes Apoptosis |
|---|---|
| المؤلفون: | Rafal Kaminski, Kamel Khalili, Armine Darbinyan, Martyn K. White, Shohreh Amini, Nana Merabova, Satish L. Deshmane, Dorota Kaniowska |
| المصدر: | Journal of Virology. 82:1558-1569 |
| بيانات النشر: | American Society for Microbiology, 2008. |
| سنة النشر: | 2008 |
| مصطلحات موضوعية: | viruses, Cellular differentiation, Immunology, JC virus, Cellular Response to Infection, Apoptosis, Biology, medicine.disease_cause, Microbiology, Cell Line, Virology, medicine, Demyelinating disease, Humans, Viral Regulatory and Accessory Proteins, Neurotropic virus, Progressive multifocal leukoencephalopathy, Oligodendrocyte differentiation, Cell Differentiation, medicine.disease, JC Virus, Cell biology, Oligodendroglia, Lytic cycle, Viral replication, Insect Science |
| الوصف: | Productive infection of oligodendrocytes, which are responsible for the formation of myelin sheath in the central nervous system, with the human neurotropic virus JC virus (JCV) causes the fatal demyelinating disease progressive multifocal leukoencephalopathy (PML). In addition to encoding T antigen and the capsid proteins, which are produced at the early and late phases of the infection cycle, respectively, JCV encodes a small regulatory protein named agnoprotein that is important for successful completion of the virus life cycle. Here we used bipotential CG-4 cells to examine the impact of agnoprotein on oligodendrocyte differentiation and survival in the absence of JCV lytic infection. We demonstrate that the expression of agnoprotein delayed the formation of complex outgrowth networks of the cells during oligodendrocyte differentiation. These alterations were accompanied by high levels of DNA damage, induction of proapoptotic proteins, and suppression of prosurvival signaling. Accordingly, apoptosis was significantly increased upon the induction of CG-4 cells toward differentiation in cells expressing agnoprotein. These observations provide the first evidence for the possible involvement of agnoprotein, independent from its role in viral replication, in a series of biological events that may contribute to the pathological features seen in PML lesions. |
| تدمد: | 1098-5514 0022-538X |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::16e80dedc9c89772caeb07df2e408f91 https://doi.org/10.1128/jvi.01680-07 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....16e80dedc9c89772caeb07df2e408f91 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 10985514 0022538X |
|---|