TREM ‐1 induces pyroptosis in cardiomyocytes by activating NLRP3 inflammasome through the SMC4 / NEMO pathway
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| المؤلفون: | Zilong Yang, Xiaoyan Pan, Xiaoxia Wu, Qiuyun Lin, Yongxia Chen, Shuting Cai, Yuanli Zhang, Zhenhua Mai, Niall Ahmad, Daqing Ma, Liehua Deng |
| المصدر: | The FEBS Journal. 290:1549-1562 |
| بيانات النشر: | Wiley, 2022. |
| سنة النشر: | 2022 |
| مصطلحات موضوعية: | Cell Biology, Molecular Biology, Biochemistry |
| الوصف: | Sepsis often causes cell death via pyroptosis and hence results in septic cardiomyopathy. Triggering receptors expressed in myeloid cells-1 (TREM-1) may initiate cellular cascade pathways and, in turn, induce cell death and vital organ dysfunction in sepsis, but the evidence is limited. We set to investigate the role of TREM-1 on nucleotide-binding oligomerization domain-like receptors with pyrin domain-3 (NLRP3) inflammasome activation and cardiomyocyte pyroptosis in sepsis models using cardiac cell line (HL-1) and mice. In this study, TREM-1 was found to be significantly increased in HL-1 cells challenged with lipopolysaccharide (LPS). Pyroptosis was also significantly increased in the HL-1 cells challenged with lipopolysaccharide and an NLRP3 inflammasome activator, nigericin. The close interaction between TREM-1 and structural maintenance of chromosome 4 (SMC4) was also identified. Furthermore, inhibition of TREM-1 or SMC4 prevented the upregulation of NLRP3 and decreased Gasdermin-D, IL-1β and caspase-1 cleavage. In mice subjected to caecal ligation and puncture, the TREM-1 inhibitor LR12 decreased the expression of NLRP3 and attenuated cardiomyocyte pyroptosis, leading to improved cardiac function and prolonged survival of septic mice. Our work demonstrates that, under septic conditions, TREM-1 plays a critical role in cardiomyocyte pyroptosis. Targeting TREM-1 and its associated molecules may therefore lead to novel therapeutic treatments for septic cardiomyopathy. |
| تدمد: | 1742-4658 1742-464X |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::16fe6e9acb42385dc5cbd47ca0a10f3c https://doi.org/10.1111/febs.16644 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....16fe6e9acb42385dc5cbd47ca0a10f3c |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 17424658 1742464X |
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