Differential inhibition of cellular and Sindbis virus translation by brefeldin A
| العنوان: | Differential inhibition of cellular and Sindbis virus translation by brefeldin A |
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| المؤلفون: | Alfredo Castello, Vanesa Madan, Iván Ventoso, Susana Molina, Miguel Angel Sanz, Luis Carrasco |
| المساهمون: | Ministerio de Economía y Competitividad (España), Fundación Ramón Areces |
| المصدر: | Digital.CSIC. Repositorio Institucional del CSIC instname |
| بيانات النشر: | Elsevier BV, 2007. |
| سنة النشر: | 2007 |
| مصطلحات موضوعية: | Sindbis virus, viruses, Eukaryotic Initiation Factor-2, Viral protein synthesis, Virus Replication, environment and public health, Cell Line, Viral Proteins, chemistry.chemical_compound, Viral envelope, Virology, Protein biosynthesis, Animals, RNA, Messenger, Replicon, Phosphorylation, Protein Synthesis Inhibitors, Brefeldin A, biology, Alphavirus Infections, Cellular translation, organic chemicals, RNA, Translation (biology), biology.organism_classification, chemistry, Protein Biosynthesis, RNA, Viral, Viral genome replication, Sindbis virus replication |
| الوصف: | Brefeldin A is a macrolide compound that interferes with the secretory pathway and also affects protein synthesis in mammalian cells. As a result, this antibiotic impedes the maturation of viral glycoproteins of enveloped viruses and viral genome replication in several virus species. In the present work, we show that translation of subgenomic mRNA from Sindbis virus, which in contrast to cellular translation is resistant to brefeldin A after prolonged treatment. The phosphorylation of eIF2 as a result of brefeldin A treatment correlates with the inhibition of cellular translation, while late viral protein synthesis is resistant to this phosphorylation. The effect of brefeldin A on Sindbis virus replication was also examined using a Sindbis virus replicon. Although brefeldin A delayed viral RNA synthesis, translation by non-replicative viral RNAs was not affected, reinforcing the idea that brefeldin A delays viral RNA replication, but does not directly affect Sindbis virus protein synthesis This study was supported by Grant (BFU2006-02182/BMC) from DGICYT and an Institutional Grant awarded to the Centro de Biología Molecular “Severo Ochoa” by the Fundación Ramón Areces |
| وصف الملف: | 179958 bytes; application/pdf |
| تدمد: | 0042-6822 |
| DOI: | 10.1016/j.virol.2007.02.001 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::170cbf234b8ebc7a7972e60be3ab9e4c |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....170cbf234b8ebc7a7972e60be3ab9e4c |
| قاعدة البيانات: | OpenAIRE |
Full Text via ClinicalKey | تدمد: | 00426822 |
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| DOI: | 10.1016/j.virol.2007.02.001 |