SUP-46 regulates the abundance of two rare tdp-1 splice junctions and SUP-46 and TDP-1 both localize to somatic nuclei. (A) Cartoon shows that, in sup-46(qa710), the rare junction between exons 3 and 5 of tdp-1 is decreased, and the rare junction between exons 5 and 6 is increased. Cartoon shows splice junctions for tdp-1. * For each junction, the number on top (bold font) is the relative abundance of that splice junction in sup-46(qa710) compared to the relative abundance in wildtype, as identified by RNASeq. Relative abundance of splice junctions was calculated by determining the number of a particular tdp-1 splice junction, as a percent of the total number of tdp-1 junctions detected for that genotype [sup-46(qa710) or wildtype]. The numbers below (grey font, in parentheses) are the total number of a particular junction detected in sup-46(qa710) (first number in pair) or wildtype (second number in pair). Splice junctions are indicated with jointed lines between exons. Rare junctions (2% or fewer of total) are indicated in red, and were altered in relative abundance in sup-46(qa710). Regions encoding the NLS and RRM domain motifs are indicated. (B) Distribution of SUP-46 and TDP-1 overlaps in somatic nuclei. Examples indicated are head neurons (blue arrow) and intestine (white arrow). Image is a composite of a live young adult hermaphrodite with the two overlapping single confocal optical sections stitched together. Scale bar represents 15 Οm. (PDF 940 kb)
