Effect of Robinia pseudoacacia Leaf Extract on Interleukin-1β–mediated Tumor Angiogenesis
| العنوان: | Effect of Robinia pseudoacacia Leaf Extract on Interleukin-1β–mediated Tumor Angiogenesis |
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| المؤلفون: | In Chul Shin, Hae Chan Ha, Yongwei Piao, Ji Min Jang, Dan Zhou, So Yoon Yun, Moon Jung Back, Hee Soo Kim, Dae Kyong Kim |
| المصدر: | In Vivo. 33:1901-1910 |
| بيانات النشر: | Anticancer Research USA Inc., 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | Cancer Research, Angiogenesis, Interleukin-1beta, General Biochemistry, Genetics and Molecular Biology, Mice, 03 medical and health sciences, 0302 clinical medicine, Western blot, Neoplasms, Tumor Microenvironment, medicine, Animals, Interleukin 8, Phosphorylation, Pharmacology, Tube formation, Tumor microenvironment, Neovascularization, Pathologic, medicine.diagnostic_test, Plant Extracts, Chemistry, NF-kappa B, Robinia, Interleukin, Molecular biology, Mice, Inbred C57BL, Plant Leaves, Vascular endothelial growth factor A, 030220 oncology & carcinogenesis, Cytokines, Ex vivo, Research Article, Signal Transduction |
| الوصف: | Background/aim Interleukin (IL)-1β is a pro-inflammatory cytokine that has recently been established as a stimulator of angiogenesis via regulation of proangiogenic factor expression in the tumor microenvironment. This study aimed to demonstrate the inhibitory effects of Robinia pseudoacacia leaf extract (RP) on IL-1β-mediated tumor angiogenesis. Materials and methods Secreted embryonic alkaline phosphatase (SEAP) reporter gene assay, ex vivo and in vitro tube formation assay, western blot, and quantitative PCR were used to analyze the inhibitory effect of RP on IL-1β-mediated angiogenesis. Results RP inhibited secretion of SEAP, blocked IL-1β signaling, and inhibited IL-1β-mediated angiogenesis in ex vivo and in vitro assays. RP inhibited nuclear translocation of NF-ĸB by suppressing phosphorylation of IL-1β signaling protein kinases and inhibited mRNA expression of IL-1β-induced pro-angiogenic factors including VEGFA, FGF2, ICAM1, CXCL8, and IL6. Conclusion RP suppressed IL-1β-mediated angiogenesis and, thus, could be a promising agent in anticancer therapy. |
| تدمد: | 1791-7549 0258-851X |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::176700ea0f37a66c433e8c98fb0ee7ae https://doi.org/10.21873/invivo.11684 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....176700ea0f37a66c433e8c98fb0ee7ae |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 17917549 0258851X |
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