Alternative Method for HDL and Exosome Isolation with Small Serum Volumes and Their Characterizations
| العنوان: | Alternative Method for HDL and Exosome Isolation with Small Serum Volumes and Their Characterizations |
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| المؤلفون: | Wisitsak Phoksawat, Patcharaporn Tippayawat, Amonrat Jumnainsong, Rian Ka Praja, Chanvit Leelayuwat |
| المصدر: | Separations, Vol 8, Iss 204, p 204 (2021) Separations Volume 8 Issue 11 |
| بيانات النشر: | MDPI AG, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | Alternative methods, Chromatography, HDL, Chemistry, Physics, QC1-999, nutritional and metabolic diseases, Filtration and Separation, Modified method, Exosome, Analytical Chemistry, small serum volume, Sample volume, biomarker, exosome, lipids (amino acids, peptides, and proteins), Protein pattern, Ultracentrifuge, isolation, QD1-999, Lipoprotein |
| الوصف: | High-density lipoprotein (HDL) and exosomes are promising sources of biomarkers. However, the limited sample volume and access to the ultracentrifuge equipment are still an issue during HDL and exosome isolation. This study aimed to isolate HDL and exosomes using an ultracentrifugation-free method with various small serum volumes. HDL was isolated from 200 µL (HDL200) and 500 µL (HDL500) of sera. Three different volumes: 50 µL (Exo50), 100 µL (Exo100), and 250 µL (Exo250) were used for exosome isolation. HDL and exosomes were isolated using commercial kits with the modified method and characterized by multiple approaches. The HDL levels of HDL200 and HDL500 were not significantly different (p > 0.05), with percent recoveries of > 90%. HDL200 and HDL500 had the same protein pattern with a biochemical similarity of 99.60 ± 0.10%. The particle sizes of Exo50, Exo100, and Exo250 were in the expected range. All isolated exosomes exhibited a similar protein pattern with a biochemical similarity of > 99%. In conclusion, two different serum volumes (200 and 500 µL) and three different serum volumes (50, 100, and 250 µL) can be employed for HDL and exosome isolation, respectively. The possibility of HDL and exosome isolation with small volumes will accelerate biomarker discoveries with various molecular diagnostic approaches. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 2297-8739 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::17ba6fb972bc569979b8acc464809a59 https://www.mdpi.com/2297-8739/8/11/204 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....17ba6fb972bc569979b8acc464809a59 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 22978739 |
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